The aim of this study is to characterize the mutations in rpoB gene of multi-drug resistant Mycobacterium tuberculosis isolates from drug resistant survey in Fujian Province, and analyze the polymorphism of alleles in rpoB gene, provid-inging the molecular evidence for rapid diagnosis for multi-drug resistant tuberculosis. The isolates were collected from the drug resistant survey sites in Fujian Province,and all strains were identified and drug susceptibility was tested. The fragment including RRDR in rpoB gene of 46 multi-drug resistant strains and 15 pan-susceptible strains were analyzed by DNA sequencing. No mutation was found in 15 pan-susceptible strains. Thirteen types of mutations (including 9 point mutations, one insertion and 3 combine mutations)in the hot-point mutant region in rpoB gene were observed in 42 multi-drug resistant strains, with a rate of mutation of 91. 30%. The sum rate of point mutations in codon 531 and that of codon 526 reached 69. 57%. The finding suggests that the most frequency point mutations in multi-drug resistant isolates from Fujian Province were codon 531 and codon 526.%目的 阐明来源于福建省耐药性调查的46株耐多药结核分枝杆菌rpoB基因的突变特征,为福建省耐多药结核病快速诊断方法的建立提供一定的分子基础.方法 对福建省9个监测点进行耐药性调查,收集菌株进行菌种鉴定、药物敏感性试验.选取46株耐多药结核分枝杆菌和15株全敏感株,扩增包含rpoB热点突变区的基因片段,测序后比对分析.结果 15株全敏感株未检测到突变.42株耐多药结核分枝杆菌rpoB基因检测到13种突变类型,涉及10个氨基酸,突变率为91.30%.突变均位于核心突变区内,包括9种点突变、1个插入突变和3种联合突变,531位和526位密码子的点突变率之和达69.57%.结论 福建省耐多药结核分枝杆菌杆rpoB基因最常见的点突变发生于531位和526位.
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