套式 PCR检测中国江苏省猪场猪鼻支原体和猪肺炎支原体的感染

摘要

目的：猪鼻支原体和猪肺炎支原体的感染在中国养猪业较为普遍，本研究对江苏省猪鼻支原体（Mycoplasma hyorhinis ，Mhr）和猪肺炎支原体（Mycoplasma hyopneumoniae ，Mhp）流行病学进行初步调查。方法从江苏省不同的猪场采集7、14、21、28及35日龄仔猪的鼻拭子样本，用套式PCR方法对收集的399份鼻拭子样本进行M hr和M hp的检测。结果江苏省不同猪场样本中M hr的检出率为70．9％，M hp检出率为13．5％。5周龄以下的猪M hr的感染率要高于M hp。其中30个样本（7．5％）的检测结果为两种病原体共感染。7-35日龄的猪M hr的感染率随着日龄的增长呈现明显的上升趋势，但M hp的感染率并未随着仔猪日龄的增长而显著上升。从本研究结果分析还发现，不同品种的猪M hr的感染率存在显著差异，与其他品种猪相比姜曲海瘦肉型品种猪（Jiangquhai porcine lean strain ，JQHPL）更易于感染Mhr （p＜0．001），但是其Mhp的感染率与其他猪相比不存在显著性差异（P＞0．05）。结论本试验调查了江苏地区猪场中仔猪感染Mhr和Mhp的情况，且研究结果表明 M hr的感染状况与日龄及品种有一定关系。%Mycoplasma hyopneumoniae (M .hyopneumoniae) and Mycoplasma hyorhinis (M .hyorhinis) infections are common in China .To investigate the prevalence of M .hyorhinis and M .hyopneumoniae in Jiangsu Province of China ,a mo-lecular epidemiological survey was conducted from 399 nasal swab samples of unvaccinated pigs using nested Polymerase Chain Reaction (nested PCR) .Nasal swab samples were collected from Jiangquhai porcine lean (JQHPL) strain pigs and other West-ern breeds .Clinical samples were taken from each pig and divided into different groups based on ages of pigs (7 ,14 ,21 ,28 , and 35 days) .Results indicated that the prevalence of M .hyorhinis was 70 .9% from different herds in Jiangsu Province in China ,while the prevalence of M .hyopneumoniae was 13 .5% .M .hyorhinis infection was more common in pigs for less than 5 weeks of age compared to M .hyopneumoniae infection .Co-infection was also observed in 30 samples (7 .5% ) in which both M .hyorhinis and M .hyopneumoniae were detected .The M .hyorhinis infection increased as the animals grew from 7 to 35 days .The M .hyopneumoniae infection did not change significantly as the pigs grew older .Significant difference of M .hyorhi-nis infection was observed between other Western breeds and JQHPL pigs (P<0 .001) .JQHPL pigs appear to be more sensi-tive to the M .hyorhinis infection as compared to the other Western breeds .However ,there is no obvious relationship between the breed type and M .hyopneumoniae infection (P>0 .05) .