猪附红细胞体 ORF5蛋白的空间结构与B 细胞抗原表位预测

摘要

The objective of the study is to predict the spatial structure and B‐cell epitopes of Mycoplasma suis ORF5 pro‐tein .The secondary structure ,hydrophilicity ,flexible region ,antigenic index and surface probability were analyzed and predic‐ted by the Protean module in DNAStar software and B Cell Epitope Prediction Tools of IDEB ,then B‐cell epitopes were predic‐ted by aggregate analysis .Results showed that the secondary structure of Mycoplasma suis ORF5 protein was relatively regu‐lar ,in which the potential B cell antigenic epitopes were located at GGVDGGRD ,GMRLPEDSR ,and EGHPDLESAR .The methods of prediction of the secondary structure and B‐cell epitopes of Mycoplasma suis ORF5 protein may provide a new method for the study of M .suis immunogenicity ,and provides a new idea for the study on immunogenicity of pathogenic micro‐organisms .%目的：预测猪附红细胞体（Mycoplasma suis ，M ．suis）ORF5基因编码蛋白的二级结构及其B细胞抗原表位。方法联合应用生物信息学软件DNAstar与在线分析软件IEDB的B Cell Epitope Prediction Tools 预测 M ．suis ORF5蛋白的二级结构及其亲水性区域、柔韧性区域、抗原指数、表面可及性等结构特征，经综合分析后预测其B细胞优势抗原表位。结果经分析可见，M ．suis ORF5蛋白具有较为规则的二级结构，潜在的优势B细胞抗原表位为GGVDGGRD、GMRLPEDSR、EGHPDLESAR氨基酸区段。结论预测出 M ．suis ORF5蛋白二级结构和B细胞抗原表位，为 M ．suis免疫原性研究提供新手段，为病原微生物免疫原性研究提供新思路。