采用反相高效液相色谱法同时测定杨梅果实中的有机酸

摘要

采用反相高效液相色谱法(RP-HPLC)研究了杨梅果实中有机酸的成分和含量,在C18柱(4.6mmID×250mm,4μm)上,采用紫外检测器(波长214 nm)进行检测,其流动相为0.04moL/L的KH2P04-H3P04缓冲液,nH2.4,流速0.8 ml/min,柱温35℃.检测所得7种有机酸标准曲线相关系数达0.999 0以上,平均回收率在88%～101%之间;变异系数RSD<3.06%.%A RP-HPLC method with a UV detector was established to determine the organic acids from the fruit of Myrica ruba.The chromatographic conditions were: Waters PhenomenexM C18(4.6 mmID×250 mm, 4 μm)used for the column(at 35 ℃)with 0.04mol·L-1 KH2PO4-H3PO4 buffer(pH2.4)as the mobile phase, and the flow rate 0.8 mL·min-1,detector wavelength UV-214 nm.The correlation coefficient of the calibration graphs was 0.999 0-0.999 9.The recovery was 88％～101％, RSD＜3.06％.