背景：中药研究骨质疏松症目前缺乏系统的药效学研究，中药组方尚欠精简，机制研究与中医理论指导不够紧密。 目的：观察中药复方龟鹿胶、淫羊藿、红景天对去卵巢大鼠血清各指标、骨密度及破骨细胞的的调控作用。 方法：建立去卵巢大鼠模型，分别用自拟中药组方龟鹿胶组、淫羊藿组、红景天组进行灌胃，以假手术组和模型组进行对比。造模后第4周开始称体质量，每周1次。造模后12周后测定大鼠骨密度、血清中钙、磷含量、血碱性磷酸酶活性等指标比较治疗效果，应用体外培养破骨细胞观察各组中药对破骨细胞抑制率的影响。 结果与结论：龟鹿胶能显著减少去卵巢大鼠体质量的增加幅度(P <0.05)。相比模型组3种中药治疗组大鼠血清中钙含量明显升高(P<0.05)，血磷与碱性磷酸酶活性明显降低(P<0.01或P<0.05)。龟鹿胶组和淫羊藿组大鼠骨密度显著高于模型组(P<0.05)。3种中药治疗组与假手术组相比吸收陷窝数明显减少(P<0.05)，其中红景天组吸收陷窝数减少较显著(P<0.01)，红景天组对破骨细胞吸收功能具有抑制作用，抑制率为86.85%(P<0.01)。结果证实，中药组方龟鹿胶、淫羊藿、红景天对去卵巢骨质疏松均具有显著疗效，其中龟鹿胶组和淫羊藿组主要以增加骨密度为主，红景天组具体表现在对破骨细胞吸收功能具有抑制作用。%BACKGROUND:At present, there are no systematic pharmacodynamic studies addressing osteoporosis treatment with Chinese medicine. Chinese prescription is not simple enough and research on the mechanism is not close to the guiding of Chinese medicine theory. OBJECTIVE:To observe the therapeutic effects of turtle deer glue, epimedium, and rhodiola pil s on serum indices, bone mineral density and osteoclast regulation in ovariectomized rats. METHODS:Ovariectomized rats were prepared and subjected to intragastric administration of turtle deer glue, epimedium and rhodiola in comparison with sham-surgery and model groups. The body mass was detected once a week starting from week 4. Bone mineral density, serum calcium, phosphorus content, and blood alkaline phosphatase activity were detected at week 12. Osteoclast inhibiting rates were evaluated by in vitro osteoclast culture model. RESULTS AND CONCLUSION:The body mass increased for the model group, the turtle deer glue could significantly reduce the increase rate (P<0.05). After 12 weeks, compared with the model group, the serum calcium content was significantly higher in the three Chinese medicine groups (P<0.05), whereas the blood phosphorus content and alkaline phosphatase activity were obviously lower (P<0.05). Bone mineral density in the turtle deer glue and epimedium groups was significantly increased as compared with that in the model group (P<0.05). The number of resorption pits was decreased significantly in the three Chinese medicine groups, especial y in the rhodiola group, as compared with the sham-surgery group (P<0.05). Rhodiola pil s could prohibit the absorptive function of osteoclasts, the inhibitory rate of which was 86.85%(P<0.01). These findings indicate that turtle deer glue, epimedium, and rhodiola al have significant effects on ovariectomized osteoporosis. Turtle deer glue and epimedium mainly increase bone mineral density, while rhodiola pil s prohibit the absorptive function of osteoclasts.
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