双螺杆挤压人参干预大鼠股骨纵向生长及力学性能改变

摘要

BACKGROUND:The twin-screw extrusion technology can alter the chemical composition of ginseng and then produce a new type of ginseng products. n OBJECTIVE:To investigate the effect of a new ginseng product, twin-screw extrusion ginseng, on the growth and biomechanics properties of the bone in rats and to discuss the possible biochemistry mechanism. n METHODS: Thirty Sprague-Dawley rats were randomly divided into control group, low-, middle-, and high-dose twin-screw extrusion ginseng groups, eight rats in each group. These rats were subjected to intragastric administration of ginseng suspension (0.75, 1.5, 3 g/kg) and distiled water for 3 weeks. After colecting the blood of heart, the femurs were taken to measure the length. Though the three-point bending test, the biomechanical properties such as the maximum bending stress, flexural modulus, bending energy of the femurs were measured. The concentration of osteocalcin and osteogenic growth peptide were tested. n RESULTS AND CONCLUSION:The bone length and concentration of osteocalcin and osteogenic growth peptide of rats in the middle-dose twin-screw extrusion ginseng group were significantly higher than those in the control group (P < 0.05). Meanwhile, the biomechanics properties of the bone in the middle-dose twin-screw extrusion ginseng group were also better than those in the control group (P < 0.05). Experimental results show that the twin-screw extrusion ginseng can promote the growth and biomechanics properties of the bone in rats, which may be relevant to the regulation of osteocalcin and osteognic growth peptide.%背景：通过双螺杆挤压技术能够使人参中的化学成分发生改变而产生新型的人参产品。n　　目的：分析新型人参加工产品双螺杆挤压人参对大鼠骨骼生长以及骨生物力学性能的影响。n　　方法：将32只SD大鼠，随机分为空白对照组和双螺杆挤压人参低、中、高剂量组，每组8只。分别灌胃蒸馏水和双螺杆挤压人参悬浊液(0.75，1.5，3 g/kg)，连续灌胃3周。心脏采血后取股骨，测量股骨长度；利用三点弯曲生物力学实验测量股骨最大弯曲应力、弯曲弹性模量、弯曲能量等生物力学性能指标。检测血液中骨钙素和成骨生长肽浓度。n　　结果与结论：中剂量双螺杆挤压人参组大鼠股骨长度、骨钙素与成骨生长肽浓度明显大于对照组(P<0.05)，同时其骨生物力学性能各项指标也明显优于对照组。结果表明双螺杆挤压人参能够促进骨骼生长且同时提升骨生物力学性能，可能与其调节骨钙素及成骨生长肽的分泌量有关。