骨桥蛋白及其受体在黄韧带骨化症黄韧带细胞中的表达及其意义

摘要

BACKGROUND:The exact pathogenesis of ossification of ligamentum flavum has not been elucidated yet. And osteopontin may be an important factor involved in the ossification of ligamentum flavum. OBJECTIVE:To clarify the expression and significance of osteopontin and its receptors, CD44 and integrin-β3, in ligamentum flavum cels between normal controls and patients with ossification of ligamentum flavum.METHODS:Ligamentum flavum tissues were obtained from normal adult controls and adult patients with ossification of ligamentum flavum (n=8 per group) who underwent thoracic/lumbar posterior decompression surgery. Ligmentum flavum cels were separated, cultured and identifiedin vitro, and osteopontin, CD44, integrin-β3 were stained using immunocytochemistry method and observed under inverted phase contrast microscope. And the mRNA expressions of osteopontin, CD44, integrin-β3 were measured by RT-PCR. RESULTS AND CONCLUSION: Immunocytochemistry results showed that the stronger positive staining for osteopontin, CD44, integrin-β3 was observed in the ossification of ligamentum flavum group than the control group (P < 0.01). The mRNA expressions of osteopontin, CD44 and integrin-β3 were also higher in the ossification of ligamentum flavum group than the control group (P < 0.05). These findings indicate that osteopontin and its receptors, CD44 and integrin-β3, in ligamentum flavum cels may play an important role in ossification of ligamentum flavum.%背景：黄韧带骨化症的机制尚不明确，骨桥蛋白可能是其骨化过程中重要的影响因素。目的：观察骨桥蛋白及其受体CD44、整合素β3在人黄韧带细胞及人骨化黄韧带细胞中的表达及其意义。方法：胸/腰椎后路减压术中取人骨化节段黄韧带组织和非黄韧带骨化黄韧带组织各8例，均来自成人，两组组织进行体外细胞分离、培养及鉴定，分别行骨桥蛋白、CD44、整合素β3免疫组织化学染色，用倒置相差显微镜观察结果，提取总RNA行半定量PCR检测。结果与结论：免疫细胞化学染色结果显示，黄韧带组织组中骨化节段黄韧带细胞骨桥蛋白、CD44、整合素β3表达强度均大于非黄韧带骨化组(P <0.01)。PCR检测结果显示，黄韧带组织组中骨化节段黄韧带细胞骨桥蛋白、CD44、整合素β3 mRNA表达强度明显强于非黄韧带骨化组(P<0.05)。结果表明，黄韧带骨化时黄韧带细胞中骨桥蛋白及其受体CD44、整合素β3基因表达均升高。