背景:鱼源胶原基医用生物材料在热稳定性、体内降解稳定性以及体内材料形态学稳定性等方面仍存在明显不足,利用技术手段提高淡水鱼类胶原海绵材料的密度和胶原分子间结合的紧密程度进而提高材料性能以满足应用需要成为拓展鱼源胶原应用领域的当务之急.目的:探究鱼皮胶原纤维重组构建纳吸棉的工艺条件.方法:以罗非鱼鱼皮为原料,在低于胶原蛋白变性温度的条件下提取酶促溶性胶原蛋白,以胶原蛋白纤维重组率为考察指标,研究胶原蛋白纤维重组工艺并对其进行参数优化,确定了鱼皮胶原纤维重组构建纳吸棉的最佳工艺.以此为基础进行重组纳吸棉的红外光谱和微观结构分析.结果与结论:通过单因素试验和正交试验得出最佳鱼皮胶原纤维重组反应条件为:胶原质量浓度为1 g/L,离子强度(NaCl浓度)为65 mmol/L,pH值7.4,体系温度为20℃,重组时间为10 h,在该条件下得到的纤维重组率高达68.6%.在此基础上制备的纳吸棉具有细线状纤维构成的多孔网状结构,且其三螺旋结构完整,是一种性能优良的腔内止血材料.%BACKGROUND: Nowadays, fish collagen biomedical materials still exhibit obvious deficiency in thermal stability,in vivo degradation stability and in vivo material morphology stability. To expand the application of fish source collagen, it is urgent to improve the material performance by increasing the density and collagen molecule tightness of freshwater fish collagen sponge materials using technique methods.OBJECTIVE: To optimize the reconstitute process for nasopore preparation using fish scale collagen.METHODS: The optimal process for nasopore preparation through the reconstitution of fish scale collagen was ascertained by taking tilapia fish skin as a raw material to extract enzymatic soluble collagen at a temperature lower than the collagen denaturation temperature and recombinant rate of collagen fibers as index. Optimization of the conditions for nasopore preparation was carried out using single factor test and orthogonal test. The prepared nasopore was analyzed through infrared spectroscopy and microstructure analysis.RESULTS AND CONCLUSION: The optimal conditions for nasopore preparation were determined through the single factor test and orthogonal test as follows: 20 ℃ for 10 hours at pH 7.4 using a mixture of 65 mmol/L NaCl and 1 g/L collagen, by which the reconstitute rate of collagen fibers was up to 68.6%. The prepared nasopore is characterized by a refined porous structure constituted by threadlike collagen fibers, and has complete three-dimensional spiral structure,which is a potential intracavitary hemostatic material with fine properties.
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