目的 探讨Survivin对人脑胶质瘤细胞经贝伐珠单抗治疗后侵袭性的影响.方法 构建抑制Survivin表达的U251/sur(-)胶质瘤细胞,经贝伐珠单抗处理.采用四甲基偶氮唑蓝法检测细胞增殖,划痕试验检测细胞的迁移能力,Transwell试验检测细胞的侵袭能力.结果 经贝伐珠单抗处理后,U251细胞的迁移、侵袭能力明显增强.与亲代U251细胞相比,抑制Survivin的U251/sur(-)细胞经贝伐珠单抗处理后,其迁移和侵袭能力明显降低.与单独抑制Survivin或单独使用贝伐珠单抗相比,将二者合用后胶质瘤细胞的增殖能力明显下降.结论 抑制Survivin可明显抑制贝伐珠单抗处理后胶质瘤细胞侵袭性增强,并与贝伐珠单抗产生协同效应,抑制胶质瘤细胞增殖.%Objective The study aims to investigate the effect of Survivin on glioma cells invasion after treated by bevacizumab.Methods Survivin shRNA plasmids were transfected in to U251 cells.The cells proliferation,migration,and invasion were determined by methyl thiazolyl tetrazolium (MTT) assay,wound healing,and transwell assay,respectively.Results The migration and invasion of U251 cells were increased by bevacizumab.Knockdown of Survivin by RNAi inhibited bevacizumab-induced cell invasion.Furthermore,the combined administration of bevacizumab and knockdown of Survivin significantly suppressed the proliferation of glioma cells compared to bevacizumab treatment or knockdown of Survivin alone.Conclusion Down-regulation of Survivin could inhibit glioma cells invasion induced by bevacizumab treatment,therefore Survivin might be a potential target to ameliorate the therapeutic efficiency of bevacizumab.
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