白念珠菌新型耐药基因IPF14744敲除质粒的构建

摘要

Objective To construct the plasmid for IPF 14744 knockout in Candida albicans . Methods Up and down streams of IPF 14744 gene ORF were amplified and inserted into the p5921 plasmid by restriction enzymes. Results The knockout plasmid Puc-14744- URA 3 was successfully constructed. Conclusions The knockout plasmid is helpful for homologous recombination knockout of IFF 14744 in Candida albicans .%目的 构建用于白念珠菌IPF 14744基因敲除的载体质粒.方法 分别扩增白念珠菌IPF 14744基因ORF两侧上下游的片段,通过酶切与连接反应,将上下游片段分别插入到p5921质粒的hisG-URA3-hisG盒两端,从而形成IPF 14744敲除载体质粒pUC-14744- URA 3.结果 成功获得IPF 14744基因敲除载体质粒.结论 所获得的质粒pUC-14744- URA3可用于白念珠菌IPF 14744基因的敲除.