A knock-out vector constructed by using YFP reporter knock-in vector and methods for preparing thereof and knocking out gene in animal cell

摘要

The present invention is a re Porter of yellow fluorescent protein LES knockout prepared using a Potter neokin vector, preparation method thereof and is to use them more particularly, relates to a method for knock out the gene in animal cells knockout vector but using the yellow fluorescent protein, knockout and character knockout vector was replaced and, fused with the promoter sequences of the knock-out gene to the initiation codon of the gene in ORF (Open Reading Frame) sequence containing the initiation codon of the yellow fluorescent protein gene by introducing the manufacturing, and the knockout vector for the animal cell relates to a method for the functions of a specific gene knockout. ; According to the present invention, in the knockout vector using the yellow fluorescent protein to re Porter, replaced the initiation codon of ATG of the knockout gene in ORF including the initiation codon of the yellow fluorescent protein, and the promoter sequence of Knockout Gene the ORF by connecting to the effect that there is a stable and highly efficient Les Porter IRES expression than conventional systems. ; Yellow fluorescent protein (YFP) Les Porter (reporter) neokin vector (knock-in vector), a knockout vector (knock-out vector), gene knockout