核酸试纸条法检测HBV基因型方法的建立

摘要

目的 建立一种快速、肉眼可观测的方法检测乙型肝炎病毒(HBV)基因型.方法 根据GenBank中已发表的明确HBV分型全序列,设计特异的引物和标记生物素探针,建立核酸试纸条法检测150例乙型患者和20例健康体检者的乙型肝炎基因型,同时采用荧光定量PCR法进行比较.结果 150例标本中B型检出率为34.00%(51/150),C型占61.33%(92/150),B/C混合型占4.00%(6/150),未分型占0.67%(1/150).与荧光定量PCR法进行比较,结果一致.结论 核酸试纸条法检测HBV基因型与荧光定量pCR法相比较灵敏度和特异性相似,但该方法更简便、快速,更适合临床开展.%Objective To develop an rapid, visuable method to detect the genotypes of HBV.Methods According to the published full-length sequence with known genotypes in GenBank, the specific primer and biotin-lablled probe were designed. We established a nucleic acid strip method for genotyping hepatitis B virus(HBV) isolates among 150 HBV infected patients and 20 healthy controls, and compared the results with those obtained by real-time PCR. Results There was 34.00% genotype B , 61.33% genotype C, 4.00% genotype B/C in 150 samples, respectively, while the remaining 0.67% unknown. The results for this assay were high comparable to the method of real-time PCR. Conclusion With the similar sensitivity and specifity when compared with real-time PCR, this rapid method is suitable to the clinical setting.