Survivin基因在急性白血病患者中的表达及其临床意义

摘要

Objective: Detection apoptosis suppressor genes Survivin in patients with acute leukemia(AL) the expression of bone marrow cells changes with AL, discussed its occurrence, development and prognosis of the relationship. Methods: Select 40 cases new-onset of acute leukemic patients as experimental and 8 cases of normal bone marrow cells as control group, using a retrovirus PCR (RT-PCR) detecting two groups of bone marrow cells Survivin mRNA expression and using immunohistochemistry SP method to detect Survivin protein expression circumstance, analyzing the acute leukemia positive and negative expression between complete remission rate (CR) difference, using a chi-square test the statistical analysis. Results: 40 cases AL patient bone marrow cells mRNA positive Survivin expression rate is significantly higher than normal control group, (65.6%,12.5%) (P＜0.05). Survivin proteins in the acute leukemia bone marrow cells of positive expression rate 65.0% (26/40) significantly higher than normal control group 12.5% (1/8) (P＜0.05), the bone marrow cells expressing negative drew Survivin after chemotherapy bone marrow complete response rate (CR) for 70.0%, obviously higher than positive convey person for CR (P＜0.05) 30.0%. Conclusion: (1) Survivin excessive expression and the occurrence of acute leukemia related to development, its positive expressions may show that the acute leukemia prognosis, (2) Survivin gene expression in leukemia cell proliferation and differentiation and (or) antiapoptotic plays an important role in the course, may be contributing to AL cells of chemotherapy drugs not sensitive one reason, Survivin gene targeting leukemia treatment can be used as a potential target.%目的 检测凋亡抑制基因Survivin 在急性白血病(AL) 患者骨髓细胞中的表达变化,探讨其与AL 的发生、发展及预后的关系.方法 选取40 例初发急性白血病患者为实验组和8例正常的骨髓细胞作对照组,采用逆转录PCR(RT-PCR) 方法检测两组骨髓细胞Survivin mRNA 的表达情况以及采用免疫组化SP 法检测Survivin 蛋白的表达情况,分析急性白血病阳性表达和阴性表达间完全缓解率(CR) 的差别,采用卡方检验进行统计学分析.结果 40 例AL 病人骨髓细胞Survivin mRNA 阳性表达率为67.5%,显著高于正常对照组12.5%(P＜0.05);Survivin 蛋白在急性白血病骨髓细胞中的阳性表达率65.0%(26/40) 明显高于正常对照组12.5%(1/8)(P＜0.05),骨髓细胞Survivin 表达阴性者化疗后骨髓完全缓解率(CR) 为70.0%,明显高于阳性表达者的CR 为30.0%(P＜0.05).结论 (1)Survivin 的过度表达与急性白血病的发生发展有关,其阳性表达可能显示急性白血病的预后不良;(2) Survivin 基因表达在白血病细胞的增殖分化和(或)抗凋亡过程中发挥了重要作用,可能是导致AL 细胞对化疗药物不敏感的原因之一,Survivin 基因可作为靶向白血病治疗的一个潜在靶点.