朝鲜红参中人参皂苷类成分含量测定及指纹图谱研究

摘要

目的：建立可同时测定朝鲜红参中人参皂苷Rb1、Rb2、Rb3、Re、Rg15种成分含量和指纹图谱的HPLC方法。方法：采用Discovery C18(4.6×250mm,5μm)柱,流动相为水(A)-乙腈(B)梯度洗脱；流速1.0ml•min-1,柱温为25℃,检测波长为203nm,并用计算机辅助相似性评价系统对指纹图谱进行了相似度分析。结果：朝鲜红参中人参皂苷Rb1、Rb2、Rb3、Re、Rg1的线性范围分别为1.077~5.385µg,1.046~5.230µg,0.0953~0.4765µg,1.018~5.090µg,0.973~4.865µg,相关系数分别为0.9999,0.9999,0.9995,0.9995,0.9996。人参皂苷Rb1、Rb2、Rb3、Re、Rg1平均回收率分别为96.55%、101.4%、99.08%、99.92%、96.00%；RSD分别为1.6%、1.2%、1.5%、1.2%、1.3%。不同规格的朝鲜红参之间及朝鲜红参与国产红参都具有较好的相关性。结论：该方法重复性好,可以用来同时测定朝鲜红参中人参皂苷Rb1、Rb2、Rb3、Re、Rg1五种成分的含量,14批不同规格的朝鲜红参中人参皂苷Rb1、Rb2、Rb3、Re和Rg15种成分含量存在差异,但总体而言,朝鲜红参与国产红参在人参皂苷含量和指纹图谱上无明显差异。%Objective:To establish a method of fingerprint and simultaneous determination of ginsenoside Rb1、Rb2、Rb3、Re and Rg1 in red ginseng from North Korea by HPLC.Methods:The column was Discovery C18 (4.6×250mm,5μm),the mobile phase consisted of water(A) and acetonitrile (B) with linear gradient elution,the flow rate was 1.0ml•min-1,the column temperature was 25℃,the detection wavelength was 203nm;the fingerprint was analysised by similar computer-assisted.Results:The linear range of ginsenoside Rb1、Rb2、Rb3、Re and Rg1 were 1.077~5.385µg (r=0.9999),1.046~5.230µg(r=0.9999),0.0953~0.4765µg(r=0.9995),1.018~5.090µg(r=0.9995),0.973~4.865µg(r=0.9996),Their average recoveries were 96.55%、101.4%、99.08%、99.92%、96.00%with RSD=1.6%、1.2%、1.5%、1.2%and 1.3%,respectively(n=5).The similarity of different sector red ginsengs from North Korea and china were good. Conclusion:The method is good reproducible and can be used to determine ginsenoside Rb1、Rb2、Rb3、Re and Rg1 in red ginseng from North Korea,the saponin contents in fourteen red ginseng from North Korea were different.There was no difference in fingerprint between red ginseng from North Korea and china.