人参中人参皂苷类成分含量测定方法优化

摘要

Objective To improve the determination method of ginsenoside Rg1 , ginsenoside Re and ginsenoside Rb1 in ginseng. Methods Four factors including extraction method, extraction solvent, solvent volume and extraction time were studied through single-factor test method. And HPLC method was adopted to analyze the determination results of ginsenoside Rg1 , ginsenoside Re and ginsenoside Rb1 . Results The best sample preparation conditions for the content determination of ginsenosides were 50 times 70% methanol, reflux extraction and duration of 2. 5 h. Conclusions The improved method is simple, accurate, and reproducible which could supply a better method for the content determination of gin-senosides.%目的：优化人参中皂苷类成分人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1含量测定方法。方法采用单因素考察方式，对提取方式、提取溶剂、溶剂倍量和提取时间4个因素进行考察，采用HPLC法测定人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1含量，分析比较含量测定结果。结果人参中皂苷类成分含量测定供试品溶液制备最佳方法为采用50倍的70%甲醇回流提取人参2.5小时。结论本实验所建立的方法操作简单、准确可靠、重现性好，为人参皂苷的含量测定提供较优方法。