中国朝鲜族六个基因座遗传多态性

摘要

Objective To investigate the genetic polymorphisms of DXS7132, DXS6854,DXS6797,DXS9898,DXS8378 and GATA31E08 short tandem repeats (STRs) loci in Chinese Korean ethnic group of Yanbian, Jilin, and to construct a preliminary database. Methods The allele frequencies of the six STRs loci in Chinese Korean ethnic individuals were analyzed by multiplex polymerase chain reaction (PCR) and polyacrylamide gel electrophoresis (PAGE). Results Total of 8, 6, 8, 8, 5 and 10 alleles were observed in each locus respectively. All loci (in female) met Hardy-Weinberg equilibrium (P>0.05). The statistical analysis of the 6 STR loci showed the heterozygosities were more than 0. 4660,the polymorphic information contents (PIC) were more than 0. 5293, the haplotype diversity were more than 0. 9993, power of discrimination (PD) in females and males were more than 0. 7737 and 0. 6107,respectively. Conclusion The results showed that, all the 6 STR loci in this study were found to have high heterozygosity and polymorphic information content, so they could provide useful markers for genetic purposes. These results could serve as valuable data to enrich the Chinese Korean ethnic group genetic database and play an important role in genetic study of Chinese population.%目的 了解中国朝鲜族群体中6个X染色体短串联重复序列(short tandem repeat,X-STR)基因座DXS7132、DXS6854、DXS6769、DXS9898、DXS8378和GATA31E08在中国朝鲜族群体中的遗传多态性.方法 用PCR扩增、变性聚丙烯酰胺凝胶电泳和银染方法对6个X-STR基因座进行检测.结果 6个基因座中分别检出8个、6个、8个、8个、5个和lO个等位基因,6个基因座基因型频率分布(女性个体)均符合Hardy-Weinberg平衡(P>0.05);观测杂合度均大于0.4660、多态信息含量均大于0.5293、女性个人识别力均大干0.7737、男性个人识别力均大于0.6107,男性单倍型多样性为0.9993.结论 6个基因座均具有较高的遗传多态性,是较理想的遗传标记系统.为中国朝鲜族起源的研究和朝鲜族群体的亲子鉴定和个人识别提供了宝贵的基础资料.