乙肝病毒基因组1896位点变异株与野生株的快速检测

摘要

Objective To establish the; rapid qualitative detration method of hepatitis B virus gennmc 1896 site vari wild strain of plasmid as standard template Werc cloned , Variant strains and wild strains HBV DNA tcmplates extrac ted from the serm samples were amplified by fluomsrence quantitative PCR. Results The experiments dctected 30 sam ples.Wild strains vere. deteeted in 7 samples, but no variant strains. Variant strains were deteeted in 4 samples, but no wild strains. Variant and wild strains of mixed infection ware deteeted in the remaining 19 samples. Conclusion This is a feasible; and rapid method to detcet hepatitis B virus genome. 1896 site variant and wild strains of qualitative methods.%目的 建立一种能同时检测乙肝病毒基因组1896位点变异株与野生株的快速检测方法.方法 设计5条特异性引物和1条特异性探针,分别克隆出含变异株与野生株的质粒作标准模板,利用荧光定量PCR检测乙肝病毒基因组1896位点变异株与野生株.结果 本次实验共检测30个标本,有7个标本只检测到野生株,4个标本只检测到变异株,其余19个标本是变异株与野生株混合感染.结论 本法是可行的能同时检测乙肝病毒基因组1896位点变异株与野生株的快速方法.