乙型肝炎病毒基因组1896位点变异株与野生株的定性检测

摘要

Objective To establish the qualitative detection method of hepatitis B virus(HBV) genome 1896 site variants and wild strains. Methods 3 strips of specific primers(consisting of 2 pairs of primers) were designed. HBV DNA templates extracted from the serum samples were amplified by PCR using 2 pairs of primers against variant strains and wild strains， and then we observed PCR products by agar gel electrophoresis. Results The experiments detected 40 samples， wild strains were detected in 11 samples， variant strains were detected in 8 samples. Variant and wild strains of mixed infection were detected in the remaining 21 samples. Conclusion This is a feasible method to detect HBV genome 1896 site variant and wild strains of qualitative methods.%目的 建立一种能同时检测乙型肝炎病毒(HBV)基因组1896位点变异株与野生株的定性方法.方法 设计3条特异性引物(组成2对引物),提取血清中HBV DNA模板进行PCR扩增变异株与野生株,取PCR产物在琼脂凝胶(含EB)上电泳,观察结果.结果 本次实验共检测40个标本,有11个标本只检测到野生株,8个标本只检测到变异株,其余21个标本都是变异株与野生株混合感染.结论 该方法是一种可行的能同时检测HBV基因组1896位点变异株与野生株的定性方法.