血栓内微泡联合尿激酶介导的超声溶栓体外实验

摘要

Objective To investigate the efficiency of intraclot microbubbles (MB) combined with urokinase (UK) mediated ultrasound (US) thrombolysis.Metho ds Fifty clots prepared by bovine whole blood were equally divided into 5 groups and were treated in a circulation system with collateral circulation tube.The clots were treated by US,MB and UK in group 1,by US and MBingroup 2,by US and UK in group 3 and by UK in group 4.The group5 was the control group without any treatment.The thrombolysis rate of each group was measured and compared.Residual clots were histologically observed with hematoxylin-eosin staining and immunofluorescence.Results The thrombolysis rate of group 1 ([73.64±14.16]％) was significantly higher than group 2 ([47.97± 11.66]％),group 3 ([57.33±8.65]％),group 4 ([50.85±9.63]％),and group 5 ([29.76±18.06] ％,all P＜0.05).Histological examination of the clots in group 1 showed multiple thrombolysis foci with clots collapse,and the degradation of fibrin network was further confirmed by immunofluorescence.Conclusion The intraclot MB mediated US thrombolysis combined with UK can enhance the rate of thrombolysis in vitro experiment.%目的 探讨血栓内微泡联合尿激酶介导的超声溶栓对体外血栓的溶解效果.方法 取牛全血制作血栓样本50个,随机平均分为5组后于带有侧支循环的体外循环装置中进行处理.实验1组为超声微泡尿激酶组,实验2组为超声微泡组,实验3组为超声尿激酶组,实验4组为单纯尿激酶组,实验5组为无处理组.计算并比较各组的溶栓率.并进行HE染色及免疫荧光组织学观察.结果 实验1组溶栓率为(73.64±14.16)％,实验2组为(47.97±11.66)％,实验3组为(57.33±8.65)％,实验4组为(50.85±9.63)％,实验5组为(29.76±18.06)％.其中实验1组溶栓率高于其余各组,差异均有统计学意义(P均＜0.05);该组HE染色组织学检查可见明显多灶状血栓溶解,边缘可见血栓崩裂,免疫荧光可见纤维蛋白网断裂.结论 血栓内微泡联合尿激酶介导的超声溶栓在体外实验中可提高血栓的溶解率.