ObjectiveTo observe the effects of different doses of Xuebijing on cerebral cortex apoptosis factors bcl-2 and bax in rats at early stage after cardiopulmonary resuscitation (CPR).Methods Thirty-two healthy SD rats aged 12 weeks were divided into four groups by using a random number table method (each,n = 8); all the rats were intubated through the opening of tracheotomy, and the blood pressure was measured through the left/right femoral artery catheter. Asphyxiation was applied to rats, resulting in cardiac arrest (CA), and then cardiopulmonary resuscitation (CPR) was carried out. After restoration of spontaneous circulation (ROSC), 0.9% normal saline 8 mL/kg, 0.9% normal saline 4 mL/kg + Xuebijing 4 mL/kg, 0.9% normal saline 2 mL/kg + Xuebijing 6 mL/kg and Xuebijing 8 mL/kg were given to model group, Xuebijing low dose group, middle dose group and high dose group respectively. The rat body weight, time of CA, CPR-ROSC time, ROSC ratio and the amounts of dopamine and 0.9% saline used in 24 hours were recorded. The positive protein expression levels of bcl-2 and bax in rat cerebral cortex and the ratio of bcl-2/bax was measured at 24 hours after ROSC.Results Compared with the model group, the amount of 0.9% normal saline (mL: 4.2±1.2, 2.6±1.0, 2.5±1.0 vs. 5.5±1.1,P < 0.05) and of dopamine used in 24 hours (μg: 1865±189, 1376±197, 1215±145 vs. 3526±141,P < 0.05), the levels of positive protein expression of bcl-2 (%: 33.4±4.3, 25.5±4.6, 26.1±4.2 vs. 38.5±5.1), and of bax at 24 hours after ROSC (%: 39.5±4.3, 32.8±3.8, 31.9±3.7 vs. 44.3±5.1) and the ratio of bcl-2/bax (0.87±0.16, 0.72±0.13, 0.71±0.14 vs. 0.89±0.11) was significantly decreased in low, middle and high dose groups (allP < 0.05). Compared with the low dose group, the amount of 0.9% normal saline and dopamine used in 24 hours, protein expression levels of bcl-2 and bax at 24 hours and the ratio of bcl-2/bax in middle dose and high dose groups were all lower than those in low dose group (allP < 0.05).Conclusion Xuebibing 6 mL/kg applied at early stage after CPR in rats may show relatively good protective effect on cerebral cortex.%目的：观察不同剂量血必净对大鼠心肺复苏（CPR）早期大脑皮质bcl-2及bax凋亡因子的影响。方法选择12周龄SD大鼠32只，按随机数字表法分为4组，每组8只。所有大鼠均先行气管切开插管，并行左/右侧股动脉置管测量血压；采用窒息法致大鼠心搏骤停（CA），然后进行CPR。模型组在自主循环恢复（ROSC）后给予0.9%生理盐水8 mL/kg；血必净低剂量组在ROSC后给予0.9%生理盐水4 mL/kg+血必净4 mL/kg；血必净中剂量组在ROSC后给予0.9%生理盐水2 mL/kg+血必净6 mL/kg；血必净高剂量组在ROSC后给予血必净8 mL/kg。记录大鼠体质量、CA时间、CPR-ROSC时间、ROSC比例、24 h多巴胺及生理盐水用量。检测ROSC后24 h时大鼠大脑皮质组织bcl-2、bax阳性蛋白表达量，并计算bcl-2/bax比值。结果与模型组相比，血必净低、中、高剂量组24 h内0.9%生理盐水用量（mL：4.2±1.2、2.6±1.0、2.5±1.0比5.5±1.1）和多巴胺用量（μg：1865±189、1376±197、1215±145比3526±141）以及bcl-2（%：33.4±4.3、25.5±4.6、26.1±4.2比38.5±5.1）、bax（%：39.5±4.3、32.8±3.8、31.9±3.7比44.3±5.1）阳性蛋白表达量和bcl-2/bax比值（0.87±0.16、0.72±0.13、0.71±0.14比0.89±0.11）均减少（均P＜0.05），血必净中、高剂量组24 h多巴胺和生理盐水用量及bcl-2、bax阳性蛋白表达量及bcl-2/bax比值均较血必净低剂量组减少（均P＜0.05）。结论 CPR后早期给予血必净6 mL/kg即可显示出较好的大脑皮质细胞保护作用。
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