细粒棘球蚴感染小鼠早期阶段Tim-3表达的初步分析

摘要

Objective:To understand the expression levels of Tim-3,a new proinflammatory factor in the early stages of Echinococcus granulosus infection in mice.Methods: BALB/c mice were infected with E.granulosus.Peritoneal macrophages and spleen cells were collected at 1,5,9 and 13 days post-infection.At different time points ,the levels of Tim-3 in peritoneal macrophages and spleen CD3+lymphocyte subsets were detected by FCM , and the relative expression of TLR 4 mRNA was detected by qRT-PCR.Results:There was no significant difference in the expression levels of Tim-3 of CD3+spleen lymphocyte subsets between E.granulosus group and control group (P>0.05).The expression levels of Tim-3 of spleen macrophages (9,13 days) and peritoneal macrophage (5,9,13 days) were much higher in E.granulosus infected group than those in control group with statistical significance (P<0.05).The numbers of macrophages were no change.Compared with control groups,the relative expression of TLR4 mRNA at 1 day post-infection was statistically higher in E.granulosus infected group ( P<0.05 ).Conclusion:During early stage of E.granulosus infection in mice,the levels of Tim-3 expression are upregulated,while the expression of TLR4 are downregulated,which may inhibit the function of macrophages resulting in host-immunity-defensive-system inhibition and immune tolerance of E.granulosus to host.%目的：了解Tim-3作为一个新型促炎因子，在细粒棘球蚴感染小鼠的早期阶段的表达水平。方法：原头蚴感染BALB／c小鼠后1、5、9、13天，取其腹腔巨噬细胞和脾细胞。采用流式细胞术（ FCM）检测各时间点腹腔巨噬细胞、脾巨噬细胞、脾CD3＋细胞Tim-3的表达水平。采用qRT-PCR法检测各时间点TLR4的mRNA水平。结果：细粒棘球蚴组与对照组小鼠CD3＋脾细胞Tim-3表达无明显差别；小鼠脾脏巨噬细胞（9、13天）、腹腔巨噬细胞（5、9、13天）表面Tim-3表达水平较对照组有升高，与对照组有显著差异（ P＜0.05）。巨噬细胞数量无明显变化。小鼠感染细粒棘球蚴第1天时TLR4 mRNA的表达水平升高，并与对照组有显著差异（ P＜0.05）。结论：小鼠感染细粒棘球蚴早期，巨噬细胞Tim-3的表达升高，并可能通过下调TLR4的表达，抑制了巨噬细胞功能，从而抑制宿主的免疫杀伤，产生了免疫逃避作用。