大黄素－BSA包被膜片人工抗原制备及其免疫原性与特异性检测

摘要

目的：通过大黄素－BSA包被膜片的免疫原性及特异性研究，探讨半抗原－载体包被膜片人工抗原制备法的可行性。方法：先将BSA包被于PVDF膜片，再与大黄素－偶联剂衍生物偶联，制备大黄素－BSA包被膜片，经膜片皮下包埋法免疫大鼠，用大黄素或大黄酚、大黄素甲醚包被CA膜片免疫分析法检测免疫原性和特异性。结果：大黄素－BSA包被膜片的免疫原性高于或等于液相抗原；其抗血清对三种蒽醌类化合物反应的特异性基本一致（ P＞0．05）。结论：大黄素－BSA包被膜片抗原，具有良好免疫原性和特异性，提示用半抗原－载体包被膜片法制备人工抗原可行。%Objective:To study feasibility of preparing artificial antigen by membrane coated with hapten-carrier.To compare the Emodin-BSA membrane antigen immunogenicity and specificity against the liquid antigen.Methods:Emodin-BSA-PVDF membrane was prepared by the method that BSA was coated on PVDF membrane and the BSA was coupled with Emodin-couplint agent derivative.Rats were immunized by subcutaneous implantation.The immunogenicity and antibody specificity were characterized using Emodin-CA or Chrysophanol-CA or Physcion-CA membrane immunoassay. Results: The immunogenicity of Emodin-BSA coated membrane antigen was higher than Emodin-BSA liquid antigen;the specificity for three anthraquinones was almost the same(P>0.05). Conclusion:Emodin antiserum generated using Emodin-BSA coated membrane antigen has a high immunogenicity and specificity to Emodin.The results show it is feasible that membrane coated with hapten-carrier is used as artificial antigen.