定坤丹对PCOS模型大鼠TGF-β1、CTGF和AR表达的影响

摘要

目的:研究定坤丹对PCOS模型大鼠TGF-β1、CTGF和AR表达的影响,探讨定坤丹治疗PCOS的作用机制.方法:应用高、中、低剂量定坤丹(4.5、2.25、1.125 g/kg)干预DHEA诱导的PCOS大鼠模型,采用ELISA、免疫组化、RT-PCR及Western blot检测各组大鼠血清激素水平、TGF-β1、CTGF和AR mRNA及蛋白的表达.结果:与模型组比较,定坤丹高、中、低剂量组E2、LH及T均下降,FSH则升高,且差异均具有统计学意义;TGF-β1、CTGF和AR mRNA及蛋白表达均下降,且差异均具有统计学意义.结论:定坤丹对PCOS大鼠有一定治疗作用,其机制可能是通过调节卵巢组织中TGF-β1、CTGF和AR的表达水平.%Objective:To investigate the effect of Ding Kundan on the expression of TGF-β1,CTGF and AR in PCOS model rats,and to explore its treat mechanism on PCOS.Methods: The PCOS model rats induced by DHEA were intervened with high, medium and low dose of Ding Kundan(4.5,2.25,1.125 g/kg),ELISA,immunohistochemistry,RT-PCR and Western blot were used to detect serum hormone levels,mRNA and protein expression of TGF-β1,CTGF and AR in each group of rats.Results: Compared with model group Ding Kundan high,medium and low dose group of E2,LH and T were decreased,while FSH was increased.And the differences were statistically significant.Protein expression and mRNA of TGF-β1,CTGF and AR were decreased,and the differences were statistically significant.Conclusion: Ding Kundan shows significantly therapeutic efficacy on PCOS rats,and its possible mechanism is related to regulating expression levels of TGF-β1,CTGF and AR in ovarian tissue.