溃疡性结肠炎患者血清低氧诱导因子-1α、CRP 的含量及其临床意义

摘要

目的：探讨溃疡性结肠炎( ulcerative colitis,UC)患者血清低氧诱导因子-1α( HIF-1α)及CRP的含量与患者病情活动性和严重性的关系。方法采用酶联免疫吸附试验( ELISA)定量检测10例健康对照者、13例缓解期UC患者、47例活动期UC患者血清中HIF-1α、CRP的含量,并对活动期UC患者行肠镜检查,评价病情轻重程度。结果活动期UC患者血清HIF-1α、CRP含量均显著高于缓解期UC患者和对照者(P<0.05),且缓解期组与对照组比较差异无统计学意义(P>0.05)；血清HIF-1α及CRP含量与病情活动性、病情分型及内镜表现分级呈正相关,且HIF-1α与CRP之间呈正相关(r=0.881, P<0.01)。结论 HIF-1α作为一种转录因子可能在UC发病机制中充当重要角色,联合检测HIF-1α及CRP可作为评价UC患者病情活动性及严重程度的良好指标。%Objective To investigate the relationship between serum hypoxia inducible factor-1 alpha ( HIF-1α) , CRP of patients and activity and severity of ulcerative colitis ( UC ) . Methods Enzyme-linked immunosorbent assay ( ELISA) was performed to detect the expression of HIF-1α and CRP in serum of patients with UC, the active UC pa-tients underwent the checking of colonoscopy and the evaluation of disease severity. Results The expression of serum HIF-1α and CRP were significantly higher in active UC patients than those in remission group and control group ( P<0 . 05 ) , and there was no significant difference between remission group and control group ( P>0 . 05 ) . The level of ser-um HIF-1αand CRP were positively correlated with disease activity, disease severity and endoscopic grading, and there was a positive correlation between HIF-1α and CRP. Conclusion HIF-1α as a transcription factor may play an impor-tant role in the pathogenesis of UC, and combined detection of HIF-1αand CRP can be used as good indexs to evaluate disease activity and severity in patients with UC.