白细胞介素(IL)-2与IL-4对小鼠膀胱癌肿瘤浸润性淋巴细胞增殖及细胞毒性的协同调节作用

摘要

Objective　To investigate the synergy effects of IL-2 and IL-4 on the proliferation and cytotoxicity of tumor-infiltrating lymphocytes (TIL) from murine bladder carcinoma in vitro.　Methods　 Suspensions of TIL were prepared from T739 murine bladder carcinoma.All cultures were incubated in RPMI-1640 complete media with IL-2 and /or IL-4. During 4 weeks of culture,TILs were counted regularly.The cytotoxicity of TILs was measured using MTT colorimetric assay.　Results　The fold expansions of TILs in IL-2 plus IL-4 for 4 weeks was 1.65 times higher than that in IL-2 alone (P<0.05).The cytotoxic activity of TIL against autologous BTT739 cells was stronger than that against YAC-1 tumor cell line at an E/T ration of 10∶1(P<0.05).IL-2 in conjunction with IL-4 could not preferentially augment the cytolysis of TIL to BTT739 cancer cells or YAC-1 tumor cell line than TIL growed in IL-2 alone did (P>0.05 ).　Conclusion　IL-4 can significantly promote the proliferation of bladder carcinoma TILs activated by IL-2, while exhibite no statistically significant effect on the cytotoxicity of TIL.%目的　探讨白细胞介素（IL）-2与IL-4对膀胱癌肿瘤浸润性淋巴细胞（TIL）体外增殖及细胞毒性免疫调控的协同作用。方法　分离膀胱癌TIL，置于含IL-2和(或)IL-4的完全培养基中培养4周，定期计数TIL增殖数量。四甲基偶氮唑蓝（MTT）比色法检测TIL细胞毒性。结果　对比单纯IL-2的培养条件，IL-2联合IL-4后4周时TIL扩增数量是前者的1.65倍（P<0.05）。在效靶比为10∶1时，TIL对自体膀胱癌细胞（BTT739）表现出高水平的杀伤活性（P<0.05）。联合培养的TIL抗BTT739 或小鼠淋巴瘤瘤株（YAC-1）的活性与在单纯IL-2培养的条件下相比无显著改变（P均>0.05）。结论　IL-4对IL-2活化的膀胱癌TIL增殖具有较强的正向调节效应，而对TIL细胞毒性未见明显影响。