北京市朝阳区2011-2017年5岁以下腹泻患儿腺病毒分子流行病学特征

摘要

Objective To investigate the genetic characteristics of human adenovirus (AdV) among infants with diarrhea in Chaoyang district,Beijing from 2011 to 2017.Methods Adenovirus positive stool samples were collected from 2011 to 2017 in Chaoyang District of Beijing.The hexon region genes of human adenovirus were sequenced.Multi-sequence alignments were performed and phylogenetic tree was constructed by Mega software.Results A total of 64 samples were sequenced and analyzed.The ratio of male to female was 11∶5.The mean age was 1.56 years.Among them,AdV41 accounted for 70.31％,followed by AdV31 (26.25％),AdV40 (4.69％),AdV1 (3.13％),AdV5 (3.13％),AdV6 (3.13％),AdV7 (3.13％),AdV2 (1.56％),AdV3 (1.56％),AdV4 (1.56％) and AdV61 (1.56％).Conclusions Human adenovirus may play an important role in viral diarrhea in Chaoyang district from 2011 to 2017.The current adenovirus epidemic is complex and AdV41 was the dominant strain in this region.%目的 研究北京市朝阳区感染性腹泻患儿中腺病毒(Adenovirus,AdV)的分子流行病学特征.方法 针对北京市朝阳区2011年4月至2017年12月收集的5岁以下腹泻患儿腺病毒感染者粪便标本,利用PCR方法检测其中腺病毒六邻体区核酸,并对扩增产物进行序列测定、腺病毒系统进化分析和型别鉴定,结合分析结果进行统计分析.结果 扩增并完成64例腺病毒阳性标本测序,男女比例为11∶5,平均年龄1.56岁,无明显的季节变化特征.系统进行分析结果显示,基于腺病毒六邻体基因,本研究共发现11种型别.AdV41型为最主要的型别,占70.31％,其次依次是AdV31型(6.25％)、AdV40型(4.69％)、AdV1型(3.13％)、AdV5型(3.13％)、AdV6型(3.13％)、AdV7型(3.13％)、AdV2型(1.56％)、AdV3型(1.56％)、AdV4型(1.56％)和AdV61型(1.56％).结论 北京市朝阳区5岁以下腹泻患儿腺病毒感染呈现出型别多样性的特征,基于腺病毒六邻体基因,AdV41型腺病毒是该地区的主要流行型别,应加强对儿童腺病毒腹泻的监测.