JY佐剂对HPV16和18型L1病毒样颗粒黏膜免疫效果的影响

摘要

Objective To evaluate the effect of JY adjuvant,which is composed of IL-2 and chitosan,on the immune response for mucosal immunization with human papillomavirus types 16 and 18 L1 virus-like particles (HPV16 + 18 L1 VLP).Methods Mice were immunized three times with HPV16 + 18 L1 VLP in the presence or absence of JY adjuvant by intramuscular and intranasal routes,respectively.Subsequently,experiments were undertaken to detect serum IgG antibody,neutralizing antibody and respiratory tract washes sIgA antibody titers and cellular immune response.Results Following intranasal immunization,serum IgG antibody titers were much higher in HPV16 + 18 L1 VLP with JY adjuvant group than that in VLP without adjuvant group (P ＜ 0.01),after intramuscular immunization,serum IgG titers induced by VLP with or without JY adjuvant were the same; following intramuscular immunization,neutralizing antibody titers induced by adjuvant-containing HPV16 + 18 L1 VLP were higher than those by adjuvant-free VLP,following intranasal immunization,only serum neutralizing antibody was detected in adjuvant-containing VLP group; after intranasal immunization,lung washes sIgA concentration were much higher in HPV16 + 18 L1 VLP with JY adjuvant group than that in VLP without adjuvant group (P ＜ 0.05) ; following intranasal and intramuscular immunization,respectively,the number of spot forming cells were much higher in HPV16 + 18 L1 VLP with JY adjuvant group than that in VLP without adjuvant group (P ＜ 0.01,P ＜ 0.05,respectively).Conclusion JY adjuvant enhanced the cellular,humoral and mocosal immunities induced with HPV16 + 18 L1 VLP by intranasal route,while showed no significant influence of the adjuvant was seen in the group immunized by intramuscular route.%目的 探讨JY佐剂对人乳头瘤病毒16和18型L1病毒样颗粒(HPV16 +18 L1 VLP)黏膜免疫效果的影响.方法 用含或不含JY佐剂的HPV16+ 18 L1 VLP分别经鼻腔和肌内注射免疫小鼠3次,检测血清IgG抗体、中和抗体和肺灌洗液sIgA抗体滴度与细胞免疫反应.结果 含JY佐剂的HPV16+ 18 L1 VLP鼻腔免疫组血清IgG抗体滴度明显高于不含佐剂组(P＜0.01),含或不含佐剂的VLP肌内注射组抗体滴度相同;含JY佐剂的HPV16+ 18 Ll VLP肌内注射免疫组血清中和抗体滴度高于不含佐剂组,仅在含有佐剂的VLP鼻腔免疫组检测到血清中和抗体;含JY佐剂的HPV16+ 18 L1 VLP鼻腔免疫组肺灌洗液sIgA抗体浓度明显高于不含佐剂组(P＜0.05);含JY佐剂的HPV16+ 18 L1 VLP鼻腔免疫和肌内注射组细胞斑点数均明显高于不含佐剂组(P ＜0.01,P＜0.05).结论 JY佐剂能增强HPV16+ 18 L1 VLP经鼻腔免疫后的小鼠的细胞、体液和黏膜免疫应答,但肌内注射免疫组佐剂作用不明显.