生长激素基因在西藏小型猪不同生长阶段和不同组织器官中的表达

摘要

Objective To study the expression of Growth Hormone ( GH) gene in different tissues and different growth stages of Tibet-minipigs.Methods The expression level of GH gene in heart, liver, spleen, lung, kidney, muscle, skin tissue of Tibet mini-pig was analyzed by Real-time PCR in 0 years old (1 days), 0.1 years old (36 days), 0.25 years old (90 days), 0.5 years old (180 days), 1 years old (360 days), 2 years old (720 days), and 3 years old (1080 days).Results The expressions of GH mRNA could be detected in various tissues (heart, liver, spleen, lung, kidney, muscle, skin) in different growth stages (0, 0.1, 0.25, 0.5, 1, 2, 3 years old) in Tibet mini-pigs.The GH gene was most highly expressed in skin in 0, 0.5 years old and most lowly expressed in muscle comparing with other tissues.Moreover, the GH gene was also most highly expressed in lung and liver in 0.25, 1 years old and 0.1, 3 years old, respectively .The expression level of GH gene reached the peak in 0.1 years old among different growth stages . Conclusion The expression of GH gene in Tibet mini-pig showed an obvious temporal and spatial specificity .%目的：对西藏小型猪生长激素基因在不同脏器和不同年龄阶段的表达水平进行测定。方法采用Real－time PCR的方法，以GAPDH为内参，定量分析0岁（1日龄）、0．1岁（36日龄）、0．25岁（90日龄）、0．5岁（180日龄）、1岁（360日龄）、2岁（720日龄）、3岁（1080日龄）的西藏小型猪的心、肝、脾、肺、肾、肌肉、皮肤组织中生长激素基因mRNA表达水平，并且进行比较分析。结果生长激素基因在各个年龄阶段的西藏小型猪的心、肝、脾、肺、肾、肌肉、皮肤中均有表达。在不同脏器的表达中，生长激素基因在0岁、0．5岁时皮肤中的表达量最高，肌肉组织中的表达量最低。在0．25岁、1岁时肺组织中表达量最高，在0．1岁、3岁时肝脏表达量最高；在不同年龄阶段的表达中，生长激素基因在0．1岁时表达达到峰值。结论西藏小型猪的生长激素基因的表达呈现出明显的时空特异性。