西藏小型猪IGF-1基因在不同生长发育阶段和不同组织器官中的差异表达

摘要

Objective To determine the expression and distribution of IGF⁃1 gene in different tissues of Tibet minipigs at different ages. Methods The changes of IGF⁃1 gene expression in different tissues and different growth stages of Tibet minipigs were detected by quantitative real⁃time fluorescence PCR technology and GAPDH gene was used as the reference. Results (1) The study of sequential expression of IGF⁃1 gene showed that the lowest expression of IGF⁃1 gene in muscle tissue was at 0, 0�25, 0�5, 1, and 2 years of age, whereas the highest expression of IGF⁃1 gene in skin tissue was at 0, 0�25, 0�5 years of age and the highest expression of IGF⁃1 gene in liver tissue was at 0�1, 2, 3 years of age. (2) IGF⁃1 gene was expressed in all the 7 examined tissues, and its expression level was in the following decreasing order:skin, lung, liver, kidney, spleen, heart and muscle at 0�25 years of age. The expression of IGF⁃1 gene in the skin was significantly higher than in other organ tissues (P<0�01). Conclusions The expression of IGF⁃1 gene of Tibet minipigs showed obvious temporal and spatial specificity.%目的：测定西藏小型猪的IGF⁃1基因在不同年龄阶段、不同组织中的表达分布情况，为未来的研究提供基础数据。方法采用实时荧光定量PCR技术，以GAPDH基因为内参照，检测了IGF⁃1基因在西藏小型猪不同组织和不同生长阶段间的表达变化。结果（1）IGF⁃1基因表达的时序性研究表明在0、0�25、0�5、1、2岁时肌肉组织的表达量最低，在0、0�25、0�5岁时皮肤组织中表达量最高，在0�1、2、3岁时肝组织的表达量最高。（2） IGF⁃1基因在在7个不同组织中均有表达，0�25岁时其在表达丰度由高到低依次为：皮肤、肺、肝、肾、脾、心脏和肌肉。且在皮肤中的表达量最高且极显著高于其他各组织（P＜0�01）。同时在不同年龄阶段的表达中，IGF⁃1基因0�25岁时在各组织中的表达均达到峰值。结论西藏小型猪的IGF⁃1基因的表达呈现出明显的时空特异性。