TIMP3基因在长白猪和通城猪不同组织及不同时期骨骼肌中的表达分析

摘要

The author performed the comparative analysis of TIMPi gene in different tissues and in skeletal muscles at different periods from Landrace and Tongcheng pigs by Quantification PCR (QPCR). The results showed that TIMP3 gene was widely expressed in all tissues examined, exhibiting similar spatial expression pattern in both pig breeds except in leg and pancreas. The gene was most highly expressed in lung and uterus, moderately expressed in skeletal muscle, spleen and stomach, and least expressed in intestine, heart. Moreover, it had been also showed that TIMP3 exhibited different expression patterns, a greater range of high-level in Tongcheng than Landrace pigs, although it showed a significant higher expression level in gestation than after birth in both pig breeds. These results suggested that TIMP3 is potentially involved in the molecular mechanisms of skeletal muscle development asynchronization and influence meat production traits in different pig breeds.%采用定量PCR(Quantification PCR,QPCR)方法,比较分析了基质金属蛋白酶3(TIMP3)基因在长白猪和通城猪间的组织表达谱,同时比较分析了其在这2个猪种胚胎期(33,45,55,65,70和90 d)和出生后(0,9,30,60,120和160 d)骨骼肌生长发育过程中的差异性表达.组织表达谱结果表明,TIMP3基因在不同猪种的各组织器官中广泛表达,除在腿肌和胰腺中有所差异外,TIMP3基因在两个猪种间的组织表达谱基本是一致的,在肺脏和子宫中表达最高,在背肌、脾脏和胃中表达较高,在肠、心脏、肾脏和肝脏中表达最低.与出生后阶段相比,TIMP3基因在2个猪种胚胎期均显著高表达(P＜0.05),但其表达变化趋势在2个猪种间存在差异；与在长白猪胚胎期相比,该基因在通城猪胚胎期中的高表达水平维持在一个更大的范围内.本试验结果提示,TIMP3基因可能参与不同类型猪种骨骼肌生长发育异步性的调控,影响到不同类型猪种的产肉性状.