外周血白细胞端粒长度与早发冠心病的相关性研究

摘要

Objective To study the correlation of telomere length of the peripheral white blood cells with premature coronary artery disease(PCAD). Methods 30 cases PCAD patients diagnosed by coronary ar-teriongraphy in our hospital were collected. At the same time , 30 cases healthy controls matching in gender and age with PCAD group were collected. PCAD patients were divided into 38~42 age group , 43~50 age group and 51~55 age group according to the age. The peripheral blood of all the subjects were collected , and the telomere length of the peripheral white blood cells were detected by southern blot. The results were analyzed statistically. Results The telomere length in PCAD group was shorter than that of control group , and the difference had statistical significance (t= 2.162,P= 0.039). There was statistical significance in the difference of telomere length among three different PCAD groups (F=4.380,P=0.020). The telomere length in 43~50 age group and 51~55 age group were all longer than that of 38~42 age group , and the differences all had statistical signifi-cance (P=0.030,P=0.020). There was no statistical significance in the difference of telomere length between 43~50 age group and 51~55 age group (P=0.090). Conclusion The telomere length of the peripheral white blood cells in patients with PCAD is obviously shorter than that of the normal controls. The telomere length of the peripheral white blood cells is correlated with PCAD.%目的：探讨早发冠心病（premature coronary artery disease， PCAD）与外周血白细胞端粒长度的相关性。方法收集我院经冠状动脉造影确诊为PCAD的患者30例，同期选取与PCAD患者性别、年龄均匹配的健康体检者30例为对照组。 PCAD患者按照年龄分为38～42岁组、43～50岁组和51～55岁组。采集受试者外周血，采用southern blot法检测外周血白细胞端粒长度，对检测结果进行统计学分析。结果 PCAD组外周血白细胞端粒长度明显比对照组短，且差异有统计学意义（t＝2.162，P＝0.039）。三个不同年龄段组PCAD患者外周血白细胞端粒长度差异有统计学意义（F＝4.380，P＝0.020）。43～50岁组和51～55岁组PCAD患者外周血白细胞端粒长度均长于38～42岁组，且差异均有统计学意义（P＝0.030，P＝0.020）；43～50岁组和51～55岁组PCAD患者外周血白细胞端粒长度差异无统计学意义（P＝0.090）。结论 PCAD患者外周血白细胞端粒长度较健康对照者明显缩短，外周血白细胞端粒长度与PCAD相关。