Purpose To explore the impact and mechanism of neural stem cells of rat on the invasiveness of C6 glioma cells of rat. Methods The cerebral cortices of newborn 1 -2 day Sprague-Dawley rats ( SD rats ) were extracted, then the neural stem cells in se-rumfree medium were separated and cultured. These cells were stained with NSE immunohistochemical staining. After that the neural stem cells were co-cultured with C6 glioma cell of rat in vitro. The invasiveness of neural stem cells of rat which is co-cultured with C6 glioma cell of rat ( the experimental group ) and individually cultured C6 glioma cell of rat ( the control group ) were detected by utilizing 2D &3D growth experiments and Transwell experiments respectively. Result In two-dimensional growth experiment, the average diameter of C6 cells in control group was ( 18. 53 ± 1. 32 ) μm, however, in experimental group it was ( 13. 44 ± 1. 45 ) μm. The difference has statistical significance( P <0. 01 ). In three-dimensional growth experiment, the average diameter of C6 cells in control group was( 20. 34 ± 1. 5 )μm, however,in experimental group it was ( 15. 52 ± 1. 43 ) μm. The difference has statistical significance ( P <0. 01 ). In the Transwell invasion experiment, the average number of membrane-spanning cells was ( 36. 45 ± 1. 36 ) in control group compared with ( 22. 73 ± 1. 66 ) in experimental group . The difference has statistical significance( P <0. 01 ). Conclusion After co-culturing with neural stem cells, the invasiveness of C6 cells decreases obviously.%目的 探讨鼠神经干细胞对鼠胶质瘤C6细胞侵袭能力的影响及机制.方法 取新生1~2天SD大鼠大脑皮层组织,于无血清培养基中分离培养神经干细胞并进行NES免疫细胞化学染色,并将神经干细胞和鼠胶质瘤C6细胞于体外共培养,利用2D、3D生长实验和Transwell实验分别检测与鼠神经干细胞共培养鼠胶质瘤C6细胞(实验组)和单独培养鼠胶质瘤C6细胞(对照组)的侵袭能力.结果 2D实验中C6细胞的对照组和实验组的平均直径分别为(18.53±1.32) μm和(13.44±1.45) μm,差异有统计学意义(P<0.01).3D实验中C6细胞的对照组和实验组的平均直径分别为(20.34±1.25) μm和(15.52±1.43) μm,差异有统计学意义(P<0.01).Transwell侵袭实验发现胶质瘤C6细胞组中对照组和实验组平均视野侵袭细胞为(36.45±1.36)个和(22.73±1.66)个,差异有统计学意义(P<0.01).结论 与神经干细胞共同培养后C6细胞的侵袭能力明显降低.
展开▼