胎盘部位滋养细胞肿瘤中NF-κBp65、CD44v6、Kiss-1的表达及相关性

摘要

Purpose To investigate the significance of the expression and correlation of NF-κBp65 , CD44v6 and Kiss-1 in placent site trophoblastic tumor ( PSTT ). Methods The expression of NF-κBp65 , CD44v6 and Kiss-1 were detected in 20 normal chorionic villis,15 hydatidiform moles, 15 chorioepitheliomas, 10 PSTTs by immunohistochemisty PowerVision method. Results There was a significant increasion of NF-κBp65 and CD44v6 expression in the tissues of chorioepithelioma and PSTT compared with that of chorionic villi and hydatidiform mole( P1 ＜ 0. 01 , P2 ＜ 0. 01 ). And there was significant different NF-κBp65 expression between PSTT and chorioepithelioma( P ＜ 0. 01 ). But no significant difference of CD44v6 were found between PSTT and chorioepithelioma( P ＞ 0. 05 ). There was a significant reduction of Kiss-1 expression in the tissues of chorioepithelioma and PSTT compared with that of chorionic villi and hydatidiform mole ( P ＜ 0. 01 ). But there was no significant difference between PSTT and chorioepithelioma( P ＞ 0. 05 ). The expressions of NF-κBp65 and CD44v6 in all groups were positively correlation( r = 0. 356, P = 0. 005) , NF-κBp65 and Kiss-1 were negatively correlation( r = - 0. 527 ,P = 0. 000 ), CD44v6 and Kiss-1 were negatively correlation( r = - 0. 346, P = 0. 007 ). Conclusions The increased expression of NF-κBp65 and CD44v6 and the decreased expression of Kiss-1 may promote the invasion of PSTT. They may play an important role in the invasion of PSTT.%目的 探讨细胞核因子-κBp65(NF-κBp65)、CD44v6、Kiss-1在胎盘部位滋养细胞肿瘤中的表达及相关性.方法 采用免疫组化PV 6000两步法检测20例正常早孕绒毛、15例葡萄胎、15例绒癌、10例胎盘部位滋养细胞肿瘤(placent site trophoblastic tumor,PSTT)中NF-κBp65、CD44v6、Kiss-1的表达情况及相关性.结果 NF-κBp65、CD44v6分别在正常早孕绒毛、葡萄胎、PSTT、绒癌中表达呈升高趋势,差异有统计学意义(P1＜0.01、P2＜0.01).NF-κBp65、CD44v6在PSTT中表达高于正常早孕绒毛和葡萄胎中的表达,差异有统计学意义(P1＜0.01、P2＜0.01、P3＜0.01、P4＜0.05);NF-κBp65在PSTT和绒癌中表达差异有统计学意义(P＜0.01);CD44v6在PSTT和绒癌中表达差异无统计学意义(P>0.05).Kiss-1在正常早孕绒毛、葡萄胎、PSTT、绒癌中表达呈下降趋势,差异有统计学意义(P＜0.01).Kiss-1在PSTT中表达低于正常早孕绒毛和葡萄胎中的表达,差异有统计学意义(P1＜0.01,P2＜0.01);在PSTT和绒癌中表达差异无统计学意义(P>0.05).NF-κBp65和CD44v6呈正相关(r=0.356,P=0.005)和Kiss-1呈负相关(r=-0.527,P=0.000);CD44v6和Kiss-1呈负相关(r=-0.346,P=0.007).结论 NF-κBp65、CD44v6的高表达和Kiss-1的低表达可能在胎盘部位滋养细胞肿瘤的浸润转移中起重要作用.