胃癌及癌前病变中COX-2和NF-κBp65蛋白表达及其相关性研究

摘要

目的 观察幽门螺杆菌(Hp)、NF-κBp65、COX-2在胃黏膜癌变过程中的表达,探讨其表达与胃癌发生、发展的关系.方法 用免疫组化方法测定病例中COX-2、NF-κBp65蛋白的表达,用亚甲兰染色检测Hp.结果 ①慢性萎缩性胃炎(CAG)、肠上皮化生(IM)、不典型增生(DYS)的Hp感染率与慢性浅表性胃炎(CSG)相比差异有显著性(x2=6.94,x2=6.71,x2=10.60,P＜0.01).胃癌(GC)的Hp感染率与CSG相比差异无显著性(x2=1.37,P＞0.05).②CAG、IM、DYS的COX-2表达率与CSG相比差异有显著性(x2 =4.81,x2=5.96,x2=16.57,P＜0.05).GC的COX-2表达率与CSG相比差异亦有显著性(x2=34.50,P＜0.01).③CAG、IM、DYS的NF-kBp65表达率与CSG相比差异有显著性(x2=4.71,x2=9.02,x2=20.74,P＜0.05).GC的NF-kBp65表达率与CSG相比差异亦有显著性(x2=30.71,P＜0.01).结论 Hp感染诱导COX-2和NF-kB表达发生在胃癌形成的早期阶段.COX-2和NF-kBp65参与了胃癌的发生发展过程,这些可能为胃癌的早期诊断及临床判断预后提供理论依据.%Objective To observe the expression of NF-kBp65 and COX-2 in gastric cancer and precancerous lesions, and to investigate the relationship between NF-kBp65 COX-2 expression and the pathogenesis of gastric cancer. Methods Slides were used for HE and sp immunohistochemical staining or Methylene Blue staining, the latter was used to detect the expression of NFkBp65, COX-2 and Hp infection. Results NF-kB and COX-2 expression is highly related with Hp infection. The COX-2 expression is positively correlated with NF-kBp65 expression (r=0. 538, P<0. 05). Conclusion The COX-2 expression is positively correlated with NF-kBp65 expression (r=0. 538, P<0. 05). NF-kBp65 and COX-2 expression is highly related with Hp infection. NF-kBp65 and COX-2 play important roles in the development progression of gastric carcinoma and NF-kBp65 may promote the expression of COX-2.