Expression and Clinical Significance of KISS-1 and TIMP-1 in Colon Carcinoma

摘要

Objective: To detect the expression of KISS-1 and mRNA TIMP-1 and protein in colon carcinoma, and to explore its clinical significance. Methods: The application of Realtime PCR was detected in 45 cases of colon carcinoma, adjacent atypical hyperplasia and normal colonic mucosa in KISS-1 and the expression of TIMP-1mRNA, SP method was used to detect KISS-1 and TIMP-1 protein expression, correlation analysis, and its occurrence and development of colon cancer invasion and metastasis and the relationship between the two. Results: The KISS-1mRNA and protein expression in normal colon mucosa, atypical hyperplasia and carcinoma were gradually decreased, there were significant differences between the groups (P < 0.05); the expression of KISS-1mRNA and protein and colon cancer invasion and lymph node metastasis (P < 0.05). The TIMP-1mRNA and protein expression in normal colon mucosa, atypical hyperplasia and carcinoma was also decreased, the difference between groups was statistically significant (P < 0.05); the expression of TIMP-1mRNA protein in colon carcinoma and invasive depth and lymph node metastasis (P < 0.05). The protein expression was positively correlated. Conclusion: The expression of KISS-1, TIMP-1mRNA and protein is closely related to the occurrence, development, invasion and metastasis of colon cancer. Colorectal cancer is one of the most common malignant tumors, the incidence increased gradually in recent years, and the age of onset is becoming younger, high mortality, research shows that the biological behavior of colon cancer. This study uses Realtime PCR and SP method in 45 cases of colon carcinoma, adjacent atypical hyperplasia and normal mucosa of colon tumor metastasis suppressor gene KISS-1 and matrix metalloproteinase inhibitors (tissue inhibitor, of metallo-proteinase1, TIMP-1) mRNA and protein expression, the objective is to investigate KISS-1 and TIMP-1 in colorectal cancer the development and invasion and metastasis of the role for the further study of colon cancer occurrence, development and invasion and metastasis mechanism and to find effective way to provide theoretical foundation of inhibiting the invasion and metastasis of colon cancer.