Au@4-硝基苯硫酚@Ag@牛血清白蛋白内标化表面增强拉曼散射探针的制备及其在细胞拉曼成像中的应用

摘要

This study explored a strategy of replacing the surface stabilizer of cetyltrimethylam-monium bromide(CTAB)on the tag surface with bovine serum albumin(BSA)and developed a BSA coated Au@ 4-nitrothiophenol(NT)@ Ag internal surface-enhanced Raman scattering (SERS)tag Au@NT@Ag@BSA. The Au@NT@Ag@BSA tag retained the monodispersity and high sensitivity of the original tag,while significantly improving the signal stability and biocom-patibility. Furthermore,labeling and Raman spectroscopic imaging of SMMC7721 lung cancer cells were realized by the incubation of the cells with the Au@NT@Ag@ BSA SERS tags. The results demonstrated that the proposed Au@NT@Ag@BSA internal SERS tags had good poten-tial for in vivo imaging applications.%制备了 Au@4-硝基苯硫酚@Ag内标化表面增强拉曼散射(SERS)探针,进一步以牛血清白蛋白(BSA)置换探针表面的稳定剂十六烷基三甲基溴化铵(CTAB),发展了 Au@NT@Ag@BSA内标化 SERS探针.Au@NT@Ag@BSA探针保留了原探针的单分散性和高灵敏度,同时显著提高了信号稳定性和生物相容性.进一步将 Au@ NT@Ag@BSA探针和 SMMC7721肺癌细胞共孵育,实现了细胞的探针标记和拉曼光谱成像.Au@NT@Ag@BSA 内标化 SERS探针在活体生物成像等方面展示了良好的应用潜力.