- Stimuli-responsive Graphene Oxide-based Polymer Brush and Metallic Nanoparticle Clusters as Inorganic-organic Composite Nanoprobes for Surface Enhanced Raman Scattering-based Biosensing and/or Bioimaging and the Method Thereof

摘要

The present invention relates to graphene oxide-based polymer brush-metal nanorod cluster organic-inorganic complexes having stimulus-responsive properties and biosensing technology based on surface enhanced Raman scattering using the same. The graphene oxide-metal nano-rod clusters for SERS-based biosensing and / or bioimage measurement according to the present invention can be obtained by rearranging graphene oxide and metal nano-rods close to each other by phase transition of NIPAA, which is a temperature- The density of the metal nanorods present on the surface of graphene oxide was increased to improve the Raman signal. In addition, the cluster-based sandwich immunoconjugate exhibited temperature reactivity as described above, and showed a linear relationship between the intensity of the Raman signal and the concentration of the molecule to be detected, indicating that the detection sensitivity was excellent. Therefore, the present invention can be used as a core technology in the fields of smart biosensing and bio-imaging.