拉曼被孢霉γ-亚麻酸高产突变株的选育

摘要

目的诱变、筛选γ-亚麻酸(GLA)高产菌株。方法采用5-氟尿嘧啶、紫外线、氯化锂复合诱变及自然分离的方法，对拉曼被孢霉AS 3.3413进行诱变筛选。结果获得一株GLA高产突变株F5。该菌株GLA产量较原始菌株提高了300%。传代实验证明该菌株遗传性质稳定。经对其发酵培养基及发酵条件的优化，该菌株GLA生产能力可达1 153.63 mg/L，较原始菌株提高335.87%。结论拉曼被孢霉GLA高产突变株F5已达工业生产菌株要求。%Purpose　The aim is to breed a highly productive γ-linolenic acid mutant strain.Methods　The original strain Mortierella ramanniance AS 3.3413 was treated by 5-FU,UV,LiCl as mutanting agent.Results　A highly productive γ-linolenic acid mutant strain F5 was successfully obtained.The γ-linolenic acid yield of mutant strain F5 was increased by 300% as compared with that of the original strain.Pass-generation test showed that the heredity character of mutant strain F5 was stable.After the optimal medium and culture condition was established, the yield of γ-linolenic acid was up to 1 153.63 mg/L,and was increased by 335.87% as compared with that of the original strain.Conclusion　The mutant strain can be used for the industrial production of GLA.