Preparation of Radiolabeled Trichothecenes, Simple Trichothecenes for Generation of Generic Antibodies, and a Study of the Fate of Trichothecenes in Soil

摘要

The objectives of this research fell into three areas: (1) preparation of radiolabeled macrocylic trichothecenes and their subsequent use in studies of trichothecene metabolism in mammalian systems; (2) preparation of a series of modified trichothecenes which will be useful in the preparation of generic antibodies for the simple trichothecenes; and (3) study of the transport in solis and plants by the trichothecenes, in order to determine the long term ecological effects of trichothecenes contamination of highly contaminated areas. 15-3H-verrucarol was prepared by two different synthetic procedures. Verrucarin A was labeled with tritium to give 2'-3H-verrucarin A and 16-3H-verrucarin A. Two nontoxic trichothecenes were prepared, one of which has a 4,15-ether bridge and the other has an 8,15-ether bridge. Roridin A was shown to bind irreversibly to soils, with the percent recovery from spiked soil sample decreasing with time and decreasing with decreasing concentration of mycotoxin in the soil. The potent cytotoxin myrotoxin B was isolated from a fungal culture and submitted for toxicological evaluation.