顺铂联合5-氟尿嘧啶对子宫内膜癌Ishikawa细胞的影响及其可能机制

摘要

Objective To investigate the effect of cisplatin and 5-fluorouracil on endocrine Ishikawa cells in the treatment of endometrial cancer. Methods The human endometrial cancer cell line Ishikawa were selected, at the logarithmic growth period, the cells were divided into three groups of 5-fluorouracil (5 mg/L) group, cisplatin (5 mg/L) group and cisplatin (5 mg/L) combined with fluorouracil (5 mg/L) group ( combined group).After treated with corresponding drugs treatment, the cell viability was detected by MTT, the apoptosis was detected by flow cytometry, and the Bcl-2 and p65 expressions cells were detected by Western blot.Results The inhibitory rate of combined group, cisplatin group and 5-fluorouracil group were (41.45 ± 3.13)%, (25.20 ±3.09)% and (23.19 ±4.10)% respectively, and the inhibitory rate in the combined group was significantly higher than that of the other two groups (P<0.05).The apoptosis rate of the combined group, cisplatin group and 5-group were (29.44 ±4.35)%, (5.74 ±1.12)% and (5.82 ±1.78)% respectively, and the apoptosis rate in the combined group was significantly higher than that of the other two groups (P<0.05).The Bcl-2 relative expression in the combined group, cisplatin group and 5-fluorouracil group were (0.31 ±0.11), (1.23 ±0.49) and (1.28 ±0.59), the p65 expression were (0.67 ±0.23), (1.67 ±0.56) and (1.71 ±0.71), combined group of Bcl-2 and p65 relative expressions were obviously less than that of the other two groups ( P <0.05 ) .Conclusion Cisplatin combined with 5-fluorouracil on endocrine Ishikawa cells in the treatment of endometrial cancer could promote cell apoptosis, inhibit the cell proliferation, and its mechanism may be related to the inhibition of Bcl-2 and p65 protein expressions.%目的：探讨顺铂联合5-氟尿嘧啶对子宫内膜癌Ishikawa细胞内分泌治疗的影响。方法将人子宫内膜癌Ishikawa细胞系进行常规传代培养，选择处于对数生长周期的细胞分为3组：5-氟尿嘧啶（5 mg／L）组、顺铂（5 mg／L）组、顺铂（5 mg／L）＋5-氟尿嘧啶（5 mg／L）组（联合组），在给予相应的药物处理后进行MTT法检测细胞抑制率和流式细胞仪检测细胞凋亡情况，同时采用免疫印迹法检测Bcl-2和p65蛋白的表达情况。结果联合组、顺铂组与5-氟尿嘧啶组的细胞抑制率分别为（41.45±3.13）％、（25.20±3.09）％和（23.19±4.10）％，联合组的抑制率明显高于其他2组（ P＜0.05）。联合组、顺铂组与5-氟尿嘧啶组的细胞凋亡率分别为（29.44±4.35）％、（5.74±1.12）％和（5.82±1.78）％，联合组的凋亡率明显高于其他2组（P＜0.05）。联合组、顺铂组与5-氟尿嘧啶组的Bcl-2相对表达量分别为（0.31±0.11）、（1.23±0.49）和（1.28±0.59），p65相对表达量为（0.67±0.23）、（1.67±0.56）和（1.71±0.71），联合组的Bcl-2和p65相对表达量都明显少于其他2组（P＜0.05）。结论顺铂＋5-氟尿嘧啶在子宫内膜癌Ishikawa细胞中的联合使用能促使细胞凋亡，抑制细胞增殖，其机制可能与抑制Bcl-2和p65蛋白的表达相关。