TPL2促进口蹄疫病毒在BHK-21细胞复制

摘要

为研究肿瘤进行性位点2(TPL2)在仓鼠肾细胞(BHK-21)中对口蹄疫病毒(FMDV)复制的影响,使用FMDV感染BHK-21细胞,通过qRT-PCR技术检测FMDV 感染对内源性 TPL2转录水平的影响;根据GenBank公布的 TPL2基因序列(NM007746.2)设计构建TPL2的真核表达质粒以及设计并合成宿主TPL2的RNAi序列,利用脂质体方法转染BHK-21细胞,通过qRT-PCR和Western blotting技术分析TPL2在BHK-21细胞过表达和干扰对FMDV复制的影响.结果表明,FMDV感染BHK-21细胞后,TPL2转录水平显著上调;过表达TPL2能够促进FMDV在BHK-21细胞中复制,而下调表达内源性TPL2后FMDV的复制受到抑制,表明TPL2促进FMDV在BHK-21细胞中进行复制,本结果进一步拓展了我们对FMDV与天然免疫机制之间关系的认识,同时为 TPL2的相关研究积累了科学资料.%This experiment was conducted to study the effects of tumor progression locus 2(TPL2)on replication of foot-and-mouth disease virus(FMDV)in baby hamster kidney(BHK-21)cells.BHK-21 cells were infected by FMDV,the mRNA level of TPL2 was detected by qRT-PCR.TPL2 eukaryotic expression plasmid and RNAi sequences were designed and synthesized based on TPL2 sequences published in GenBank(NM007746.2).The effects of TPL2 on FMDV replica-tion in BHK-21 cells were evaluated by qRT-PCR and Western blotting.The results indicated that the transcriptional level of TPL2 was significantly up-regulated during FMDV infection ;Overexpression of TPL2 facilitated the replication of FMDV and downregulation of endogenous TPL2 inhibited FMDV replication.These results revealed that TPL2 promoted the proliferation of FMDV in BHK-21 cells.This research further expanded our understanding of the relationship between FMDV and the innate immune mechanism and had accumulated scientific data for the rel-evant research of TPL2.