hTERT基因反义核酸对TNF-α抗SCID小鼠氟它胺耐受型前列腺癌影响机制研究

摘要

目的 建立SCID小鼠氟它胺耐受型前列腺癌模型,探讨hTERT基因反义核酸(AS PS-ODN)对肿瘤坏死因子-α(TNF-α)抗肿瘤的影响机制.方法 建立氟它胺耐受型前列腺癌模型,体内传代至其生物学特性趋于稳定,观察TNF-α对肿瘤细胞LNCaP-flu的抑制作用.结果 hTERT基因AS PS-ODN联合TNF-α治疗组肿瘤生长明显抑制,SCID小鼠生存时间延长,生活质量优于单用TNF-α组.结论 hTERT AS PS-ODN能降低SCID小鼠氟它胺耐受型前列腺癌细胞LNCaP-flu端粒酶活性;对TNF-α抗肿瘤有协同作用.%Objective To investigate the effect of human telomerase reverse transcriptase (hTERT) antisense on tumor necrosis factor-a (TNF-α)-induced apoptosis in flutamide insensitive (androgen-independent) prostate cancer cells (LNCaP-flu). Methods Flutamide insensitive animal model was established in SCID mice. The growth of tumor was observed in flutamide insensitive prostate cancer animal model treated with hTERT AS PS-ODN and TNF-α treatment. Results The growth of tumor was inhibited obviously, survival time of the SCID mice was prolonged and life quality was improved after hTERT AS PS-ODN combined with TNF-α treatment. Conclusion Inhibition of telomerase with hTERT antisense can enhance TNF-α-induced apoptosis of flutamide insensitive prostate cancer cells in SCID mice.