兔煤工尘肺模型各期HRCT表现与血清TNF-α、PDGF表达水平相关研究

摘要

Objective:To investigate the association of the HRCT features and expression of serum TNF-α and PDGF in the rabbits model of coal worker's pneumoconiosis (CWP). Methods:Copying the CWP rabbit model,at 2 weeks and month of 1,2,3,5,CT scan was performed in each group,meanwhile,levels of TNF-α and PDGF in serum were measured by ELISA test. Results:HRCT features in the CWP rabbits model showed that ground-glass opacities and thick interlobular septa were observed in the early stage,pulmonary fibrosis and small honeycomb imaging were found in the middle stage,nodules、extensive ring shadow and opacities were discovered in the late stage. The level of TNF-α in serum of CWP rabbits was lower,but the level of PDGF was higher in comparison with those of the control (P<0.05,respectively). Moreover,with the pulmonary lesions aggra-vating,serum TNF-α and PDGF decreased gradually. TNF-α expression level was positively related to PDGF (P<0.05). TNF-α、PDGF expression levels were negatively related to the severity of pulmonary interstitial fibrosis (P<0.05). Conclusion:The expres-sion levels of TNF-α and PDGF in serum maybe related to the severity of pulmonary interstitial fibrosis,TNF-α and PDGF play an important role.%目的:探讨兔煤工尘肺模型在HRCT上的影像表现与肿瘤坏死因子-α(TNF-α)、血小板源性生长因子(PDGF)表达水平的相关性. 方法:复制兔煤工尘肺模型,分别于2周、1、2、3、5个月时对兔行HRCT扫描,同时采用酶联免疫吸附法(ELISA)检测兔血清TNF-α、PDGF含量. 结果:煤工尘肺模型兔肺HRCT早期主要表现为磨玻璃影及小叶间隔增厚,中期主要表现为局限性肺间质纤维化及小蜂窝状影像,晚期主要表现为结节影、广泛环状影及实变影;试验组兔血清TNF-α含量低于对照组,差异有统计学意义(P<0.05),PDGF含量高于对照组,差异有统计学意义(P<0.05),且随时间的延长,TNF-α、PDGF水平均逐渐降低;两变量相关性分析显示TNF-α与PDGF呈正相关(P<0.05),TNF-α、PDGF含量变化与兔煤尘肺模型HRCT表现严重程度之间呈负相关(P<0.05). 结论:煤工尘肺高分辨力CT表现与TNF-α、PDGF表达水平密切相关,TNF-α、PDGF在肺组织不可逆纤维化过程中均发挥重要作用.