Objective To explore the effects of ganoderma lucidum polysaccharides ( GLP) on the immune function of macrophages in mice bearing human ladder cancer T 24 cells.Methods Nude mice model bearing the human bladder cancer T 24 cells was established from May 2012 to October 2014, mice were divided into three groups by random number table method:0.9%sodium chloride solution group , cisplatin group and cisplatin +GLP group, there were 5 mice in each group.Mice in the above three groups received intragastric administration of 0.9% sodium chloride solution , 0.9% sodium chloride solution and GLP, respectively , and received intraperitoneal injection of 0.9% sodium chloride solution , cisplatin and cisplatin , respectively.The peritoneal macrophages were then collected , the phagocytic rate of peritoneal macrophages against FITC -dextran was further detected by flow cytometry , the levels of IL-1 and TNF-αin peritoneal macrophages were measured by ELISA, and real-time fluorescent quantitative polymerase chain reaction ( qPCR) was used to analyze the mRNA levels of IL-1 and TNF-αin peritoneal macrophages.Results The positive rates of FITC -dextran, CD11b -FITC and CD68 -FITC among mice in cisplatin+GLP group were 4.5%, 11.5%and 13.4%higher than those among mice in cisplatin group , respectively , the above indicators among mice in cisplatin +GLP group and cisplatin group were lower than those among mice in 0.9%sodium chloride solution group , respectively.There were significant differences in levels of IL-1 and TNF-α, expression levels of IL-1 mRNA and TNF-αmRNA in peritoneal macrophages among three groups of mice ( P <0.05 ); levels of IL-1 and TNF-α, expression levels of IL-1 mRNA and TNF-αmRNA in peritoneal macrophages among mice in cisplatin +GLP group and cisplatin group were significantly lower than those among mice in 0.9%sodium chloride solution group , respectively ( P<0.05); the above indicators among mice in cisplatin +GLP group were significantly higher than those among mice in cisplatin group ( P<0.05 ) .Conclusion GLP could improve the phagocytic function of peritoneal macrophages in mice , thus can improve immune function of body , the mechanism of which may be related to the enhancement effect of GLP on IL-1 and TNF-αexpression levels.%目的：探讨灵芝多糖对荷膀胱癌T24细胞小鼠化疗后腹腔巨噬细胞免疫功能的影响。方法2012年5月—2014年10月建立荷膀胱癌T24细胞小鼠模型15只，采用随机数字表法分为3组：0.9％氯化钠溶液组、顺铂组、顺铂+灵芝多糖组，每组5只。分别以0.9％氯化钠溶液、0.9％氯化钠溶液、灵芝多糖灌胃18 d，以0.9％氯化钠溶液、顺铂、顺铂腹腔注射5d。收集小鼠腹腔巨噬细胞，采用流式细胞仪检测腹腔巨噬细胞对异硫氰酸荧光素-右旋糖酐（FITC-dextran）的吞噬能力，以FITC-dexran阳性率表示；采用酶联免疫吸附试验法（ELISA）检测腹腔巨噬细胞白介素1（IL-1）和肿瘤坏死因子α（TNF-α）表达水平；采用荧光定量PCR （RT-PCR）检测腹腔巨噬细胞IL-1 mRNA和TNF-αmRNA表达水平。结果顺铂+灵芝多糖组小鼠FITC-dextran阳性率较顺铂组升高4.5％；顺铂+灵芝多糖组小鼠CD11b -FITC阳性率和CD68-FITC阳性率分别较顺铂组升高11.5％和13.4％，但均未能达到0.9％氯化钠溶液组巨噬细胞中阳性率。3组小鼠腹腔巨噬细胞IL-1、 TNF-α表达水平及IL-1 mRNA、 TNF-αmRNA表达水平比较，差异有统计学意义（ P＜0.05）；其中顺铂组和顺铂+灵芝多糖组小鼠腹腔巨噬细胞IL-1、 TNF-α表达水平及IL-1 mRNA、 TNF-αmRNA表达水平较0.9％氯化钠溶液组降低（ P＜0.05）；顺铂+灵芝多糖组小鼠腹腔巨噬细胞IL-1、 TNF-α表达水平及IL-1 mRNA、 TNF-αmRNA表达水平较顺铂组升高（ P＜0.05）。结论灵芝多糖能够提高荷膀胱癌T24细胞小鼠腹腔巨噬细胞的吞噬作用，从而提高机体的免疫功能，其机制可能与增强IL-1和TNF-α的表达水平有关。
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