灵芝多糖对荷膀胱癌T24细胞小鼠T细胞亚群及AQP1、AQP3表达的影响

摘要

目的：研究灵芝多糖对荷膀胱癌T24细胞小鼠T细胞亚群及AQP1、AQP3表达的影响。方法选择BALB／c裸鼠30只作为实验动物，皮下注射膀胱癌T24细胞建立荷膀胱癌动物模型，随机分为对照组、顺铂组、灵芝多糖＋顺铂组各10只，顺铂组给予顺铂25 mg／kg腹腔注射，顺铂＋灵芝多糖组小鼠给予灵芝多糖200 mg／kg灌胃，顺铂25 mg／kg腹腔注射，对照组给予相当体积的生理盐水灌胃。测定肿瘤组织的体积和质量、外周血中T细胞亚群的含量以及肿瘤组织中AQP1、AQP3、Caspase-3、Bax的mRNA含量。结果顺铂组、顺铂＋灵芝多糖组小鼠肿瘤组织的体积、肿瘤组织质量明显低于对照组（P＜0.05），顺铂＋灵芝多糖组小鼠肿瘤组织体积、肿瘤组织质量明显低于顺铂组（P＜0.05）；顺铂组、顺铂＋灵芝多糖组CD4＋T细胞、CD8＋T细胞、CD4＋／CD8＋均明显高于对照组（P＜0.05），顺铂＋灵芝多糖组CD4＋T细胞、CD4＋／CD8＋均明显高于顺铂组（P＜0.05）；顺铂组、顺铂＋灵芝多糖组小鼠肿瘤组织中Bax、Caspase-3的mRNA含量明显低于对照组（P＜0.05），顺铂＋灵芝多糖组鼠肿瘤组织中Bax、Caspase-3的mRNA含量明显低于顺铂组（ P＜0.05）；顺铂组、顺铂＋灵芝多糖组小鼠肿瘤组织中AQP1、AQP3的mRNA含量明显低于对照组（ P＜0.05）；顺铂＋灵芝多糖组小鼠肿瘤组织中AQP1、AQP3的mRNA含量明显低于顺铂组（ P＜0.05）。结论灵芝多糖干预能够抑制荷膀胱癌T24细胞小鼠的肿瘤生长，增强细胞免疫功能，调节凋亡基因、AQP1、AQP3的表达水平。%Objective To study the effect of ganoderma lucidum polysaccharides on T cell subsets and AQP1, AQP3 expression of bladder cancer T24 cell line bearing mice.Methods 60 BALB/C nude mice were selection as experimental animals, bladder tumor bearing animal models were made by bladder cancer T24 cells subcutaneous injection, and were randomly divided into control group, cisplatin group, ganoderma lucidum polysaccharides and cisplatin group, cisplatin group given 25 mg/kgcisplatin intraperitoneal injection, cisplatin combined ganoderma lucidum polysaccharide group given 200 mg/kg ganoderma lucidum polysaccharide lavage, 25 mg/kg cisplatin intraperitoneal injection, the control group given quite a volume normal saline lavage.Then tumor volume and weight, peripheral blood T cell subsets and AQP1, AQP3, Caspase-3, Bax mRNA content in tumor tissue were determined.Results Cisplatin group, cisplatin combined ganoderma lucidum polysaccharide tumor volume and mass were significantly lower than the control group (P<0.05), cisplatin and ganoderma lucidum polysaccharide tumor volume and mass were significantly lower than that cisplatin group ( P<0.05 ); Cisplatin group, cisplatin combined ganoderma lucidum polysaccharides group CD4 + T cells, CD8 +T cells , CD4 +/CD8 + were significantly higher than that of control group (P<0.05), cisplatin combined ganoderma lucidum polysaccharide group CD4 +T cells, CD4 +/CD8 +were significantly higher than that of cisplatin group (P<0.05); Cisplatin group, cisplatin combined ganoderma lucidum polysaccharide group mices tumor tissues Bax, Caspase 3 mRNA content were significantly lower than the control group (P<0.05), cisplatin combined ganoderma lucidum polysaccharide group Bax,caspase 3 mRNA content were significantly lower than that of cisplatin group (P<0.05); Cisplatin group, cisplatin combined ganoderma lucidum polysaccharide group mice tumor tissue AQP1, AQP3 mRNA content were significantly lower than the control group (P<0.05); Cisplatin combined ganoderma lucidum polysaccharide group AQP1, AQP3 mRNA content were significantly lower than cisplatin group (P<0.05 ). Conclusion ganoderma lucidum polysaccharide can inhibit tumor growth and enhance cellular immune function of bladder cancer T24 cell line bearing mice, and adjust the expression of pro-apoptotic genes, AQP1 and AQP3.