目的:分析核酸实验室试剂损耗的原因,对存在的问题分析以及改进,以达到减少损耗的目的,降低检测成本.方法:对广州血液中心2014年334 492人份核酸试剂检测有效报告数和试剂损耗数进行统计,分析试剂损耗的各种因素,探讨对减少试剂损耗的措施.结果:2014年消耗334 492人份核酸试剂,发布有效检验报告数294 465人份,试剂利用率88.03%,总消耗率11.97%,包括日常质控检测损耗率9.32%,系统故障率1.89%,鉴别实验损耗率0.74%以及其他损耗0.02%.结论:影响试剂损耗的原因有日常质控检测的消耗,系统的硬件、软件的故障,鉴别实验使用,人为的操作失误等其他多方面的因素.其中日常质控检测的消耗,系统的硬件、软件的故障是最主要因素.%Objective:To analyze the causes of the loss of nucleic acid laboratory reagent,analyze the problems and improve the reagent,so as to achieve the purpose of reducing loss,and reduce the detection cost.Method:334 492 reagents for nucleic acids detection report number and reagent loss number in the Guangzhou blood center in 2014 were counted,reagent loss factors were analyzed,the measures to reduce the loss of reagent were explored.Result:In 2014,334 492 nucleic acid reagents were consumed,the number of effective test report was 294 465,the utilization rate of the reagent was 88.03%,the total consumption rate was 11.97%,including the consumption of the daily quality control rate 9.32%,the system failure rate 1.89%,the identification of the experimental use rate 0.74% and other loss rate 0.02%.Conclusion:The cause of the reagent loss has the consumption of daily quality control,the hardware and software of the system,the fault of the software,the identification of the experimental use,the human error and other factors.The consumption of the daily quality control,the hardware and software of the system are the main factors.
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