西妥昔单抗对肺腺癌SPC-A-1细胞放射增敏作用研究

摘要

目的 探讨西妥昔单抗(C225)联合放射治疗对肺腺癌SPC-A-1细胞的放射增敏作用.方法 成克隆细胞形成实验分为对照组(6MV-X线照射)和实验组(6MV-X线照射+C225),分别给予0、1、2、4、6、8Gy照射后培养10天,计数≥50个细胞的克隆数.采用多靶单击模型进行数据处理,拟合细胞存活曲线,计算细胞增敏比(SER).用Hoechst 33258染色观察细胞凋亡情况,流式细胞术检测细胞凋亡率.结果 成克隆细胞形成实验结果显示,扣除C225毒性影响后实验组的存活分数比对照组低(P＜0.05),SERD0为1.42.Hoechst 33258染色荧光显微镜下观察,实验组细胞核呈波纹状,染色质浓缩.细胞凋亡实验结果显示,对照组和实验组0、2、4、8Gy的凋亡率分别为(4.45±0.45)%、(11.09±1.03)%、(20.30±0.70)%、(23.91±0.37)%和(11.00±1.42)%、(20.10±1.58)%、(36.90±1.88)%、(40.41±1.15)%(P＜0.05).结论 C225对SPC-A-1细胞系有放射增敏作用,其机制可能与诱发细胞凋亡有关,该结果为临床综合治疗肺癌提供了理论基础.%Objective To explore the radiosensitivity of cetuximab (C225), an anti-epidermal growth tactor receptor monoclonal antibody, combined with irradiation against human non-small lung cancer cell line SPC-A-1.Methods SPC-A-1 cells were processed with different doses of 6-MV X-ray including 0,1,2,4,6,8Gy alone or together with C225 (100nmoL/L).The courtenay assay was showed by the surival curve.Apoptosis analysis was detected by flow cytometry assay.Results Compared with the irradiation alone group, the clone number in combination group was significantly decreased (P ＜0.05).The sensitizing enhance rate(SER) of Do was 1.42.Under Hoechst 33258 staining fluoescence microscopy, the nuclei were corrugated and chromatin was clumped in treatmant group.The percentage of apoptotic SPC-A-1 cells in 0,2,4 and 8 Gy irradiation alone groups was ( 4.45 ± 0.45 ) ％, ( 11.09 ± 1.03 ) ％, ( 20.30 ± 0.70 ) ％ and ( 23.91 ± 0.37 ) ％, which were lower than ( 11.00 ± 1.42 ) ％, ( 20.10 ± 1.58 ) ％, ( 36.90 ± 1.88 ) ％ and(40.41 ± 1.15 ) ％ in each irradiation dose combined with C225 prosessment group ( P ＜ 0.05 ).Conclusion The mechanism of radiosensitivity enhancement of C225 to SPC-A-1 cells may induce to apoptosis.This result provides a support for clinical combined treatment in lung cancer.