目的:建立同时测定补阳还五汤中芍药苷、毛蕊异黄酮苷、阿魏酸含量的方法.方法:采用反相高效液相色谱法.色谱柱为OdyssilC18(250 mm×4.6 mm,5 μm),流动相为乙腈-水-0.1％磷酸(16∶64∶20,V/V/V),检测波长为230 nm(芍药苷)、260 nm(毛蕊异黄酮苷)、328 nm(阿魏酸),流速为1.0 ml/min,柱温为30℃.结果:芍药苷、毛蕊异黄酮苷、阿魏酸的进样量分别在0.096 4～0.964 0 μg(r=0.999 2)、0.099 0～0.990 0 μg(r=0.999 6)、0.008 36～0.083 6 μg(r=0.999 4)范围内与各自峰面积积分值呈良好线性关系；三者的平均加样回收率分别为99.72％、99.14％和97.96％,RSD分别为0.5％、1.1％和1.4％(n均为6).结论:本方法简便、快速、灵敏度高、重复性好,适用于补阳还五汤的质量控制.%OBJECTIVE: To establish the method for simultaneous determination of paeoniflorin, calycosin and ferulic acid in Buyang huanwu decoction. METHODS: RP-HPLC method was adopted. The determination was performed on Odyssil C18(250 mm× 4.6 mm,5 μm) column with mobile phase consisted of acetonitrile-water-0.1% phosphate acid (16 : 64:20, V/V/V) at the flow rate of 1.0 ml/min. The detection wavelength was set at 230 nm (paeoniflorin), 260 nm (calycosin) and 328 nm (ferulic acid). The column temperature was 30 ℃. RESULTS: The linear ranges were 0.096 4-0.964 0 μg(r=0.999 2) for paeoniflorin, 0.099 0-0.990 0 μg for calycosin (r=0.999 6) and 0.008 36-0.083 6 μg for ferulic acid(r=0.999 4),respectively. The average recoveries were 99.72% for paeoniflorin (RSD=0.5% ,n=6) ,99.14% for campanulin(RSD=l.l% ,n=6) and 97.96% for ferulic acid (RSD=1.4%,n= 6). CONCLUSION: The method is simple, quick, sensitive and reproducible for the quality control of Buyang huanwu decoction.
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