目的：观察茶多酚对酒精性肝损伤大鼠肝脏的抗炎与抗氧化作用。方法将36只SD大鼠适应性喂养1周后，随机分为对照组、模型组及治疗组，各12只。对照组大鼠每日用生理盐水按8 g/kg灌胃，模型组大鼠给予同剂量酒精灌胃，治疗组大鼠在灌胃同时按0.25 g/kg剂量灌胃给予茶多酚。经过8周后，检测大鼠血清丙氨酸氨基转移酶（ALT）、天冬门氨酸氨基转移酶（AST）、谷胱甘肽巯基转移酶（GST）活性及肝脏中超氧化物歧化酶（SOD）、丙二醛（MDA）、谷胱甘肽过氧化物酶（GSH－Px）含量，以及肝脏中炎症相关因子白细胞介素（IL）－6、IL－1β、单核细胞趋化蛋白－1( MCP－1)、肿瘤坏死因子－α( TNF－α)表达。结果长期酒精灌胃喂养可明显使大鼠诱发酒精性肝损伤，服用茶多酚后可明显降低酒精性肝损伤大鼠增高的血清中ALT等活性，并能减少肝组织中MDA含量，升高SOD与GSH－Px的活性，并降低IL－6和IL－1β等的表达。结论茶多酚对大鼠酒精性肝损伤具有抗炎和抗氧化的保护作用。%Objective To observe the anti-inflammatory and anti-oxidative protection effect of tea polyphenols on the alcoholic liver injury rats. Methods 36 SD rats feed adaptive after 1 week,then randomly divided into the normal control group,model group and treatment group,12 cases in each group. Rats in normal group infused with normal saline once a day with a dose of 8 g/kg body weight,the model group infused with the same dose of alcohol,the treatment infused with the alcohol and tea polyphenols with a dose of 0. 25 g/kg body weight. After 8 weeks,the ALT,AST and GST levels in serum and SOD,MDA and GSH-Px levels in liver tissue and the expression of IL-6,IL-1β,MCP-1 and TNF-α of rats were detected. Results Long-term gastric feeding method by alco-hol can obviously make alcohol induced liver injury in rats. After treating with tea polyphenols,the higher serum ALT,AST and GST activity obviously down-regulated in alcoholic liver injury rats,and the MDA level was reduced,the SOD activity increased,the expres-sion of IL-6,IL-1β,TNF-α and MCP-1 were decreased. Conclusion Tea polyphenols has anti-inflammatory and anti-oxidant protection on alcoholic liver injury rats.
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