乳脂中1,3-甘油二酯检测方法的研究

摘要

A qualitative and quantitative determination of the 1,3 - diacylglycerol( 1,3 - DG) in milk fat was established. The 1,3 - DG was effectively separated and qualitatively identified by TLC , and then was quantitatively analyzed by GPO - PAP. The RSD of the method was no more than 3.78％ and the recovery was 86.73％ - 101.83 ％ 。 The method was accurate, precise, rapid and suitable for the analysis of 1,3 - DG in milk fat.%建立一种乳脂中1,3-甘油二酯(1,3-DG)定性定量的检测方法.通过薄层色谱法(TLC)对乳脂中1,3-甘油二酯进行分离和定性,再用甘油氧化酶法进行定量检测.研究结果显示:薄层色谱法能有效分离出1,3-甘油二酯;定量方法RSD小于等于3.78%,回收率在86.73%～101.83%之间.该方法能准确、快速、灵敏地分离检测乳脂中的1,3-甘油二酯.